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改良 ELISA 用于抗体亲和力评估：标准化的必要性。

Modified ELISA for antibody avidity evaluation: The need for standardization.

机构信息

Adolfo Lutz Institute, Immunology Division, Sao Paulo, Brazil; Sao Paulo University, Biomedical Sciences Institute, Post-Graduation Program Interunity in Biotechnology, Sao Paulo, Brazil.

Adolfo Lutz Institute, Immunology Division, Sao Paulo, Brazil.

出版信息

Biomed J. 2021 Aug;44(4):433-438. doi: 10.1016/j.bj.2020.10.009. Epub 2020 Oct 27.

DOI:10.1016/j.bj.2020.10.009
PMID:34493482
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8515003/
Abstract

Antibody avidity is an important parameter to evaluate immune response, being useful to evaluate vaccine responses and helping to distinguish acute and latent infection. The antibody avidity can be measured by different methods, yet the most common is a modified ELISA. The utilization of commercial kits or in-house methods to evaluate antibody avidity have been adopted more and more, although the lack of standardization between different assays may generate a lot of variation in the process, making it hard to compare the results generated.

摘要

抗体亲合力是评估免疫反应的一个重要参数，有助于评估疫苗反应，并有助于区分急性感染和潜伏感染。抗体亲合力可以通过不同的方法来测量，最常用的是改良的 ELISA 法。越来越多的人采用商业试剂盒或内部方法来评估抗体亲合力，尽管不同检测方法之间缺乏标准化可能会导致该过程产生大量差异，从而难以比较生成的结果。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b67/8515003/b28357d486a5/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b67/8515003/b28357d486a5/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b67/8515003/b28357d486a5/gr1.jpg

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