Nixon Susan, Sieg Anke, Delgado-Charro M B, Guy Richard H
Novartis Consumer Health SA, Nyon, Switzerland.
J Pharm Sci. 2007 Dec;96(12):3457-65. doi: 10.1002/jps.20989.
This work investigates the reverse iontophoretic extraction of lactate, a widely used marker of tissue distress in critically ill patients and of sports performance. In vitro experiments were performed to establish the relationship between subdermal lactate levels and lactate iontophoretic extraction fluxes. Subsequently, the iontophoretic extraction of lactate was performed in vivo in healthy volunteers. Lactate was quickly and easily extracted by iontophoresis both in vitro and in vivo. During a short initial phase, iontophoresis extracts the lactate present in the skin reservoir, providing information of relevance, perhaps, for dermatological and cosmetic applications. In a second step, lactate is extracted from the interstitial subdermal fluid allowing local lactate kinetics to be followed in a completely non-invasive way. The simultaneous in vivo extraction of chloride, and its possible role as an internal standard to calibrate lactate reverse iontophoretic fluxes, was also demonstrated. Despite these positive findings, however, considerably more research is necessary to eliminate potential artefacts and to facilitate interpretation of the data.
本研究探讨了反向离子电渗法提取乳酸的过程，乳酸是危重症患者组织损伤和运动表现的常用标志物。进行了体外实验以建立皮下乳酸水平与乳酸离子电渗提取通量之间的关系。随后，在健康志愿者体内进行了乳酸的离子电渗提取。无论是在体外还是体内，离子电渗法都能快速、轻松地提取乳酸。在最初的短时间内，离子电渗法提取皮肤储库中的乳酸，这可能为皮肤科和化妆品应用提供相关信息。在第二步中，从皮下组织间液中提取乳酸，从而能够以完全无创的方式跟踪局部乳酸动力学。同时还证明了体内氯离子的提取及其作为校准乳酸反向离子电渗通量内标物的可能作用。然而，尽管有这些积极的发现，但仍需要进行大量研究以消除潜在的假象并便于数据解释。
