Xiao Chenwen, Rajput Zahid Iqbal, Hu Songhua
Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou 310029, China.
Vaccine. 2007 Jun 15;25(25):4795-800. doi: 10.1016/j.vaccine.2007.04.027. Epub 2007 Apr 26.
In model animal experiment A, ICR mice were immunized with ovalbumin (OVA) adjuvanted with Quil A or mineral oil or their combination (Quil A+oil). In model animal experiment B, ICR mice were immunized with foot-and-mouth disease virus (FMDV) antigens or with a commercially available oil adjuvanted foot-and-mouth disease (FMD) vaccine (type O) alone or mixed with Quil A. After that, serum samples were collected to analyze specific IgG and IgG subclasses IgG1, IgG2a, IgG2b, and IgG3. In experiment C, pigs were immunized with FMD (type O) vaccine alone or together with Quil A. Serum samples were collected before and 4 weeks after immunization to analyze indirect haemagglutination (IHA) titers. Results from experiment A indicated a synergistic effect of Quil A and oil on IgG and the subclass responses. Experiment B revealed that supplement of Quil A in FMD vaccine significantly increased IgG and the subclass responses in mice. Experiment C demonstrated that supplement of Quil A in the FMD vaccine significantly enhanced humeral immune responses (as determined by IHA test) in pigs. It is concluded that supplement of Quil A in FMD vaccine can significantly enhanced immune responses and could be an alternative way to improve FMD vaccination in pigs.
在模型动物实验A中，用含Quil A或矿物油或其组合（Quil A+油）佐剂的卵清蛋白（OVA）免疫ICR小鼠。在模型动物实验B中，用口蹄疫病毒（FMDV）抗原或单独使用市售油佐剂口蹄疫（FMD）疫苗（O型）或与Quil A混合免疫ICR小鼠。之后，收集血清样本以分析特异性IgG和IgG亚类IgG1、IgG2a、IgG2b和IgG3。在实验C中，用FMD（O型）疫苗单独或与Quil A一起免疫猪。在免疫前和免疫后4周收集血清样本以分析间接血凝（IHA）效价。实验A的结果表明Quil A和油对IgG及亚类反应具有协同作用。实验B显示在FMD疫苗中添加Quil A可显著增加小鼠的IgG及亚类反应。实验C表明在FMD疫苗中添加Quil A可显著增强猪的体液免疫反应（通过IHA试验测定）。得出的结论是，在FMD疫苗中添加Quil A可显著增强免疫反应，并且可能是改善猪FMD疫苗接种的一种替代方法。
