Zhang Qi, Mulder Jan, Truin Gert-Jan, van Palenstein Helderman W H
Key Laboratory for Oral Biomedical Engineering of Ministry of Education, Department of Endodontics, School and Hospital of Stomatology, Wuhan University, Luoyu Road 237, Wuhan, Hubei Province 430079, China.
J Dent. 2007 Jul;35(7):588-92. doi: 10.1016/j.jdent.2007.04.002. Epub 2007 May 16.
This study aimed to test the following hypotheses: (1) a carry-over effect of EC40 does not occur in a split-mouth model, (2) two consecutive applications of EC40 varnish with an interval of 3 months per year has a better inhibiting effect on mutans streptococci in pit and fissure plaque of permanent first molars of 6-7-year olds than a repeated application at each 6 months and (3) EC40 exerts an inhibiting effect on mutans streptococci for at least a 6-month duration.
Using a split-mouth design, test molars received EC40 on fissures and pits and control molars remained without treatment. One group of test molars received EC40 at baseline, 6, 12 and 18 months and another group at baseline, 3, 12 and 15 months. Plaque samples were taken from pits and fissures of test and control molars at baseline, 6, 9, 12 and 24 months. A mixed ANOVA model was used to analyze the data.
The first hypothesis was accepted, the second rejected and the third accepted.
Twice a year EC40 application on pits and fissures of permanent first molars of 6-7-year-old children can significantly reduce mutans streptococci numbers for a 6-month period, but the reductions were small.
本研究旨在验证以下假设：（1）在半口模型中不会出现EC40的残留效应；（2）每年间隔3个月连续两次应用EC40清漆，对6至7岁儿童恒牙第一磨牙窝沟菌斑中变形链球菌的抑制效果优于每6个月重复应用一次；（3）EC40对变形链球菌的抑制作用至少持续6个月。
采用半口设计，试验磨牙的窝沟接受EC40处理，对照磨牙不做处理。一组试验磨牙在基线、6个月、12个月和18个月时接受EC40处理，另一组在基线、3个月、12个月和15个月时接受处理。在基线、6个月、9个月、12个月和24个月时从试验和对照磨牙的窝沟采集菌斑样本。使用混合方差分析模型分析数据。
第一个假设被接受，第二个被拒绝，第三个被接受。
每年两次在6至7岁儿童恒牙第一磨牙的窝沟应用EC40可在6个月内显著减少变形链球菌数量，但减少幅度较小。
