Ilkit Macit, Hilmioglu Suleyha, Tasbakan Meltem, Aydemir Sohret
Department of Microbiology, Faculty of Medicine, University of Çukurova, Adana, Turkey.
Department of Microbiology, Faculty of Medicine, University of Ege, Izmir, Turkey.
J Med Microbiol. 2007 Jun;56(Pt 6):762-765. doi: 10.1099/jmm.0.46625-0.
In this study, 250 vaginal samples from patients with vulvovaginal candidosis were inoculated onto two chromogenic media, Albicans ID(2) and Biggy agar, as well as onto Sabouraud chloramphenicol agar, yielding a total of 63 yeast (25.2 %) on all three media. These strains were identified as Candida glabrata in 20 (31.8 %) samples, Candida albicans in 15 samples (23.8 %), Candida tropicalis in 10 samples (15.9 %), Candida krusei in five samples (7.9 %), Candida kefyr in five samples (7.9 %), Candida dubliniensis in four samples (6.3 %), Candida parapsilosis in two samples (3.2 %) and Candida guilliermondii in two samples (3.2 %). Mixed fungal cultures and bacterial growth or filamentous fungi were not detected on any of the selected media. The sensitivity and specificity of the Albicans ID(2) and Biggy agar with regard to the identification of C. albicans were 80.0 and 64.6 %, and 86.7 and 56.3 %, respectively. This study showed these two chromogenic media to be as effective as Sabouraud chloramphenicol agar with respect to fungal detection. However, neither Albicans ID(2) nor Biggy agar was sufficient for reliable differentiation of yeasts to the species level.
在本研究中，将250份来自外阴阴道念珠菌病患者的阴道样本接种到两种显色培养基（白色念珠菌鉴定培养基（2）和Biggy琼脂）以及沙氏氯霉素琼脂上，在所有三种培养基上共培养出63株酵母菌（25.2%）。这些菌株在20份样本（31.8%）中被鉴定为光滑念珠菌，15份样本（23.8%）中为白色念珠菌，10份样本（15.9%）中为热带念珠菌，5份样本（7.9%）中为克鲁斯念珠菌，5份样本（7.9%）中为凯菲念珠菌，4份样本（6.3%）中为都柏林念珠菌，2份样本（3.2%）中为近平滑念珠菌，2份样本（3.2%）中为季也蒙念珠菌。在所选用的任何培养基上均未检测到混合真菌培养物、细菌生长或丝状真菌。白色念珠菌鉴定培养基（2）和Biggy琼脂在鉴定白色念珠菌方面的敏感性和特异性分别为80.0%和64.6%，以及86.7%和56.3%。本研究表明，这两种显色培养基在真菌检测方面与沙氏氯霉素琼脂同样有效。然而，白色念珠菌鉴定培养基（2）和Biggy琼脂都不足以将酵母菌可靠地鉴别到种水平。
