Baradkar V P, Mathur M, Kumar S
Department of Microbiology, Lokmanya Tilak Municipal Medical College & General Hospital, Sion, Mumbai 400 002, India.
Indian J Pathol Microbiol. 2010 Jan-Mar;53(1):93-5. doi: 10.4103/0377-4929.59192.
Chromogenic media are frequently used in direct and rapid identification of yeasts because different Candida species produce unique colors on these media. We used 60 isolates of Candida species including 30 C. albicans, 10 C. parapsilosis, 11 C. glabrata, five C. tropicalis, and four C. dubliniensis, isolated from various clinical specimens, to evaluate the performance of HiChrome Candida agar. These strains had been identified by germ tube test, morphology on cornmeal agar, chlamydospore formation on tobacco agar and sugar assimilation tests. The sensitivity and specificity results were: C. albicans (96.55 and 96.42%); C. parapsilosis (80 and 98.03%), C. glabrata (90.90 and 88.23%), C. tropicalis (100 and 100%) and C. dubliniensis (60 and 96.55%) respectively. HiChrom Candida agaris medium has been useful and capable of presumptive, rapid identification of Candida species within 48 hours.
显色培养基常用于直接快速鉴定酵母菌，因为不同念珠菌属在这些培养基上会产生独特的颜色。我们使用了从各种临床标本中分离出的60株念珠菌，包括30株白色念珠菌、10株近平滑念珠菌、11株光滑念珠菌、5株热带念珠菌和4株都柏林念珠菌，来评估HiChrome念珠菌琼脂的性能。这些菌株已通过芽管试验、玉米粉琼脂上的形态学、烟草琼脂上的厚垣孢子形成以及糖同化试验进行了鉴定。敏感性和特异性结果分别为：白色念珠菌（96.55%和96.42%）；近平滑念珠菌（80%和98.03%），光滑念珠菌（90.90%和88.23%），热带念珠菌（100%和100%）和都柏林念珠菌（60%和96.55%）。HiChrom念珠菌琼脂培养基很有用，能够在48小时内对念珠菌属进行初步的快速鉴定。
