Maier Johannes K X, Balabanian Sylvia, Coffill Cynthia R, Stewart Alexandra, Pelletier Louise, Franks Douglas J, Gendron Nathalie H, MacKenzie Alex E
Department of Biochemistry, Microbiology, and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada.
J Histochem Cytochem. 2007 Sep;55(9):911-23. doi: 10.1369/jhc.6A7144.2007. Epub 2007 May 17.
The neuronal apoptosis inhibitory protein (NAIP) gene, also known as the baculovirus inhibitor of apoptosis repeat-containing protein 1 (BIRC1) gene, is a member of the inhibitors of apoptosis (IAP) family and was first characterized as a candidate gene for spinal muscular atrophy (SMA). The expression of NAIP has been thoroughly studied in the central nervous system and overlaps the pattern of neurodegeneration in SMA. Recent studies have pointed to a role for NAIP in non-neuronal cells. We report here the production of a specific anti-NAIP antibody and the profile of NAIP expression in human adult tissues by Western blot and immunohistochemical detection methods. NAIP was detected in a number of tissues by Western blot analysis, but immunohistochemistry revealed that NAIP's presence in certain tissues, such as liver, lung, and spleen, is most likely due to macrophage infiltration. In the small intestine, the expression of NAIP coincides with the expression of p21(WAF1). This observation, coupled with findings from other groups, suggests a role for NAIP in increasing the survival of cells undergoing terminal differentiation as well as the possibility that the protein serves as an intestinal pathogen recognition protein. This manuscript contains online supplemental material at http://www.jhc.org. Please visit this article online to view these materials.
神经元凋亡抑制蛋白（NAIP）基因，也被称为含杆状病毒凋亡重复序列蛋白1（BIRC1）基因，是凋亡抑制蛋白（IAP）家族的成员，最初被鉴定为脊髓性肌萎缩症（SMA）的候选基因。NAIP的表达已在中枢神经系统中得到深入研究，且与SMA中的神经退行性变模式重叠。最近的研究指出NAIP在非神经元细胞中发挥作用。我们在此报告了一种特异性抗NAIP抗体的产生，以及通过蛋白质印迹法和免疫组织化学检测方法在成人组织中NAIP的表达情况。通过蛋白质印迹分析在多种组织中检测到了NAIP，但免疫组织化学显示NAIP在某些组织（如肝脏、肺和脾脏）中的存在很可能是由于巨噬细胞浸润。在小肠中，NAIP的表达与p21（WAF1）的表达一致。这一观察结果，结合其他研究小组的发现，提示NAIP在增加终末分化细胞的存活率方面发挥作用，以及该蛋白可能作为肠道病原体识别蛋白的可能性。本文包含在线补充材料，网址为http://www.jhc.org。请在线访问本文以查看这些材料。
