Department of Biochemistry and Molecular Biology II, School of Pharmacy, University of Granada, 18071, Granada, Spain.
Institute of Nutrition and Food Technology "José Mataix", Biomedical Research Center, Avda. del Conocimiento S/N, Armilla, 18016, Granada, Spain.
J Mol Histol. 2021 Feb;52(1):113-123. doi: 10.1007/s10735-020-09928-y. Epub 2020 Nov 25.
The neuronal apoptosis inhibitory protein (NAIP) is a constituent of the NLRC4 inflammasome, which plays a key role in innate immunity, and an antiapoptotic protein. Recently, we reported the previously undescribed role of NAIP in cell division. The liver is one of the body's most actively regenerative organs. Given the novel mitotic role of NAIP, we examined its expression in hepatic mass restoration. The major liver lobe of Wistar rats was removed, and samples from both newly formed liver tissue, assessed by positive Ki67 immunostaining, and the remnant, intact liver lobes from hepatectomized rats were taken 3 and 7 days after surgery. Naip5 and Naip6 mRNA levels were significantly higher in regenerating hepatic tissue than in intact liver lobe tissue, and this increase was also observed at the protein level. Naip5 and Naip6 mRNA in situ hybridization showed that this increase occurred in the hepatic parenchyma. The histology of the regenerated liver tissue was normal, with the exception of a noticeable deficiency of hepatic lobule central veins. The results of this study suggest the involvement of NAIP in liver mass restoration following partial hepatectomy.
神经元凋亡抑制蛋白（NAIP）是 NLRC4 炎症小体的组成部分，在先天免疫中发挥关键作用，也是一种抗凋亡蛋白。最近，我们报道了 NAIP 在细胞分裂中的先前未描述的作用。肝脏是人体最活跃的再生器官之一。鉴于 NAIP 的新型有丝分裂作用，我们检查了其在肝质量恢复中的表达。Wistar 大鼠的主要肝叶被切除，分别取自手术后 3 天和 7 天的新形成的肝组织（通过阳性 Ki67 免疫染色评估）和肝叶切除大鼠的剩余完整肝叶的样本。在再生的肝组织中，Naip5 和 Naip6 mRNA 水平明显高于完整的肝叶组织，并且在蛋白质水平上也观察到这种增加。Naip5 和 Naip6 mRNA 的原位杂交显示，这种增加发生在肝实质中。再生肝组织的组织学正常，除了肝小叶中央静脉明显缺乏。这项研究的结果表明，NAIP 参与了部分肝切除后的肝质量恢复。
