Frank M, Dapson Rw, Wickersham Tw, Kiernan Ja
Biological Stain Commission, Department of Pathology, University of Rochester Medical Center, Rochester, NY, USA.
Biotech Histochem. 2007 Feb;82(1):35-9. doi: 10.1080/10520290701333475.
Nuclear fast red (CI 60760), also known as Kernechtrot, is commonly used in conjunction with an excess of aluminum ions as a red nuclear counterstain following histochemical procedures that yield blue products. The dye has also been used as a histochemical and colorimetric reagent for calcium. Unsatisfactory samples of nuclear fast red are encountered occasionally, and confusion has resulted from applying the name of the dye to neutral red (CI 50040), an unrelated compound with different properties. Tests for the identity and performance of nuclear fast red have been developed in the laboratory of the Biological Stain Commission. The Commission will now accept samples submitted by vendors for certification. We describe here the spectrophotometric, chromatographic and biological staining methods that are used to identify and test nuclear fast red.
核固红（CI 60760），也称为固红，通常与过量铝离子一起用作组织化学程序产生蓝色产物后的红色核复染剂。该染料还用作钙的组织化学和比色试剂。偶尔会遇到核固红的不合格样品，并且由于将该染料的名称应用于中性红（CI 50040）（一种具有不同性质的无关化合物）而导致混淆。生物染色委员会实验室已开发出核固红的鉴定和性能测试方法。委员会现在将接受供应商提交的样品进行认证。我们在此描述用于鉴定和测试核固红的分光光度法、色谱法和生物染色方法。
