Bishop Michael J, Crow Brian, Norton Dean, Kovalcik Kasey, George Joe, Bralley J Alexander
Metametrix Clinical Laboratory, R&D 201, 4855 Peachtree Industrial Blvd., Norcross, GA 30092, USA.
Rapid Commun Mass Spectrom. 2007;21(12):1920-4. doi: 10.1002/rcm.3044.
An analytical method was developed for the rapid and accurate quantification of leucine (LEU) and isoleucine (ILE) from plasma using electrospray ionization tandem mass spectrometry (ESI-MS/MS). The two isomeric amino acids were selectively detected using fragment ions unique to each compound. As a result, the need for chromatographic separation was avoided allowing for faster analysis (3 min). The possibility of any considerable interference between the two transitions was closely monitored, with no significant interference being observed. The presence of interfering compounds in plasma was also evaluated and found to minimal. The method was evaluated based upon linearity, with r2>or=0.995 for both compounds, and accuracy, with no more than 8% deviation from the theoretical value.
开发了一种使用电喷雾电离串联质谱（ESI-MS/MS）从血浆中快速、准确地定量亮氨酸（LEU）和异亮氨酸（ILE）的分析方法。利用每种化合物特有的碎片离子选择性地检测这两种同分异构氨基酸。结果，避免了色谱分离的需要，从而实现了更快的分析（3分钟）。密切监测了两个跃迁之间任何显著干扰的可能性，未观察到明显干扰。还评估了血浆中干扰化合物的存在情况，发现其干扰极小。该方法根据线性进行评估，两种化合物的r2≥0.995，根据准确性进行评估，与理论值的偏差不超过8%。
