Daikoku Shusaku, Ako Takuro, Kurimoto Ayako, Kanie Osamu
Mitsubishi Kagaku Institute of Life Sciences, Machida-shi, Tokyo, Japan.
J Mass Spectrom. 2007 Jun;42(6):714-23. doi: 10.1002/jms.1196.
The majority of structural investigations of oligosaccharides based on mass spectrometry use naturally occurring oligosaccharides, which do not allow extracting any common feature associated with anomeric structures and linkage positions. In order to address the issue to find such characteristics possibly contained in oligosaccharide structure, a synthetic combinatorial trisaccharide library was analyzed. The trisaccharides used in the analysis consisted of L-fucose, D-galactose and D-glucose, in which individual glycosidic linkages existed in either alpha- or beta-anomers. The analysis of energy-resolved mass spectra (ERMS) and the scattered plot analysis of some parameters obtained from ERMS for a series of trisaccharides revealed that lower activation energy was required for the dissociation of alpha-glycosides of these sugars compared to those of the corresponding beta-anomers. It is suggested that this finding may be useful in structural analysis of natural oligosaccharides.
大多数基于质谱的寡糖结构研究使用天然存在的寡糖，这无法提取与异头物结构和连接位置相关的任何共同特征。为了解决寻找寡糖结构中可能包含的此类特征这一问题，对一个合成组合三糖文库进行了分析。分析中使用的三糖由L-岩藻糖、D-半乳糖和D-葡萄糖组成，其中各个糖苷键以α-或β-异头物形式存在。对一系列三糖的能量分辨质谱（ERMS）分析以及从ERMS获得的一些参数的散点图分析表明，与相应的β-异头物相比，这些糖的α-糖苷解离所需的活化能更低。有人认为这一发现可能对天然寡糖的结构分析有用。
