Kurono S, Ohashi Y, Hiruma K, Okinaga T, Hoshi M, Hashimoto H, Nagai Y
Glycobiology Research Group, Institute of Physical and Chemical Research (RIKEN), Saitama, Japan.
J Mass Spectrom. 1998 Jan;33(1):35-44. doi: 10.1002/(SICI)1096-9888(199801)33:1<35::AID-JMS607>3.0.CO;2-I.
Fast atom bombardment mass spectrometry (FABMS) and collision-induced dissociation tandem mass spectrometry (CID-MS/MS) were applied to the investigation of the anomeric isomerism of synthetic trisaccharides consisting of xylose, galactose and sulfated fucose {Xyl1-->3Gal alpha 1-->3(4-OSO3Na)Fuc} and {Xyl1-->3Gal alpha 1-->4(3-OSO3Na)Fuc} and the linkage position of the sulfate group. It was possible to differentiate between various glycosidic linkages in several synthetic trisaccharides. The position of a sulfate group in synthetic methyl O-sulfo-alpha-L-fucopyranoside isomers was elucidated from the fragmentation patterns. Comparing the data from the synthetic sulfated trisaccharides with the spectra from the natural compound derived from glycan chains of the acrosome reaction-inducing substance (ARIS) from starfish, the anomeric structure and the position of the sulfate group in the natural sample were determined without ambiguity as Xyl beta 1-->3Gal alpha 1-->3(4-OSO3-)Fuc, in agreement with the result from an independent study based on nuclear magnetic resonance.
快速原子轰击质谱法（FABMS）和碰撞诱导解离串联质谱法（CID-MS/MS）被用于研究由木糖、半乳糖和硫酸化岩藻糖组成的合成三糖{Xyl1→3Galα1→3(4-OSO3Na)Fuc}和{Xyl1→3Galα1→4(3-OSO3Na)Fuc}的端基异构现象以及硫酸根的连接位置。在几种合成三糖中区分不同的糖苷键是可行的。从裂解模式阐明了合成甲基O-磺基-α-L-岩藻吡喃糖苷异构体中硫酸根的位置。将合成硫酸化三糖的数据与来自海星顶体反应诱导物质（ARIS）聚糖链的天然化合物的光谱进行比较，天然样品中的端基结构和硫酸根位置被明确确定为Xylβ1→3Galα1→3(4-OSO3-)Fuc，这与基于核磁共振的独立研究结果一致。
