Ma Ying-Li, Ma Chao
School of Pharmacy, Heilongjiang University of Traditional Chinese Medicine, Harbin 150040, China.
Zhongguo Zhong Yao Za Zhi. 2007 Mar;32(5):418-20.
To establish a HPLC method for the determination of diosmin in Rats plasma and to study the pharmacokinetics of diosmin in Rats.
Rats were given diosmin with 3 doses as 225, 325, 425 mg x kg(-1). Blood samples were collected at different times after oral administration. The plasma concentration of diosmin was determined by HPLC, and the pharmacokinetics parameters were calculated by 3p97 program.
The typical equation of diosmin in rats plasma was Y = 3.05 x 10(-3) C + 1.55 x 10(-3), the calibration curves of diosmin was linear in the range from 0.5-100 microg x mL(-1) (R =0. 996 4). The lowest concentration of diosmin in plasma was 0. 2 g x mL(-1). Its recoveries was more than 85%, and the interday and intraday precision, which was expressed as RSD, were all less than 15%. After 3 doses oral administration of diosmin in rats, the mean plasma concentration-time curves were found to fit one compartment model, and the main pharmacokinetics parameters were obtained.
It is first time to establish the HPLC method to determine the concentration of diosmin in rats plasma, and the method described in this report has high sensitivity and selectivity, and it was suitable for pharmacokinetics studies of diosmin. The internal process of diosmin in rats is fit to one compartment model.
建立测定大鼠血浆中地奥司明的高效液相色谱法，并研究地奥司明在大鼠体内的药代动力学。
给大鼠分别灌胃225、325、425mg·kg⁻¹ 3种剂量的地奥司明。口服给药后于不同时间点采集血样。采用高效液相色谱法测定血浆中地奥司明的浓度，并用3p97程序计算药代动力学参数。
大鼠血浆中地奥司明的典型方程为Y = 3.05×10⁻³C + 1.55×10⁻³，地奥司明的标准曲线在0.5 - 100μg·mL⁻¹范围内呈线性（R = 0.996 4）。血浆中地奥司明的最低检测浓度为0.2μg·mL⁻¹。其回收率大于85%，以相对标准偏差（RSD）表示的日间和日内精密度均小于15%。大鼠口服3种剂量地奥司明后，平均血浆浓度 - 时间曲线符合一室模型，并获得了主要药代动力学参数。
首次建立了测定大鼠血浆中地奥司明浓度的高效液相色谱法，本报告所述方法具有高灵敏度和选择性，适用于地奥司明的药代动力学研究。地奥司明在大鼠体内的过程符合一室模型。
