Yang Yong-Qing, Yang Gong-She
Laboratory of Fat Deposition and Muscle Development, North West Sci-tech University of Agriculture and Foristry, Yangling 712100, China.
Zhongguo Zhong Yao Za Zhi. 2007 Mar;32(5):424-7.
To study the effect of emodin (EMO) on the proliferation and differentiation of rat preadipocytes.
Separating and culturing rat preadipocytes, grouping the wells that preadipocytes were growing according to certain concentration such as 0, 5, 10, 20, 40, 80, 160 micromol x L(-1) randomly, MTT spectrophotometry and flow cytometry (FCM) were adopted to determine the effect of EMO on proliferation of rat preadipocytes. The accumulation of TG (triglyceride) in adipocytes was assayed by oil red O staining, and the morphological changes of the adipocytes were determined by morphology observation.
EMO in the range of 20-160 micromol x L(-1) could inhibit the proliferation and differentiation of preadipocytes in a dose and time dependent manner, and induce apoptosis of preadipocytes in a certain degree.
EMO should have a potential to serve as a fat-reducing drug.
研究大黄素（EMO）对大鼠前体脂肪细胞增殖和分化的影响。
分离培养大鼠前体脂肪细胞，将前体脂肪细胞生长的孔按0、5、10、20、40、80、160微摩尔×升（-1）等一定浓度随机分组，采用MTT分光光度法和流式细胞术（FCM）检测EMO对大鼠前体脂肪细胞增殖的影响。用油红O染色法检测脂肪细胞内甘油三酯（TG）的蓄积情况，通过形态学观察确定脂肪细胞的形态变化。
20 - 160微摩尔×升（-1）范围内的EMO可呈剂量和时间依赖性抑制前体脂肪细胞的增殖和分化，并在一定程度上诱导前体脂肪细胞凋亡。
EMO有作为减肥药物的潜力。
