Poitout V, Moatti D, Velho G, Reach G, Sternberg R, Thévenot D R, Bindra D S, Zhang Y N, Wilson G S
INSERM U 341, Service de Diabételogie, Paris, France.
ASAIO Trans. 1991 Jul-Sep;37(3):M298-300.
A miniaturized glucose sensor was developed, consisting of a platinum wire entirely coated with teflon, except for a 1 mm section near its extremity where glucose oxidase is immobilized. The in vitro sensitivity to glucose of the sensors was 2.3 +/- 0.4 nA/mM, mean +/- SEM (n = 23). These sensors were implanted in the subcutaneous tissue of normal beagles. Two consecutive glucose infusions (15-30 mg/kg/min) were performed. The current generated by the sensor was used for calculation of the sensitivity coefficient (SC) (nA/mM), and the background current in the absence of glucose (lo) (nA). These parameters were used for determination of the apparent subcutaneous glucose concentration. The in vivo sensitivity was less than the in vitro sensitivity (0.5 +/- 0.1 vs. 2.2 +/- 0.2; n = 12 comparisons; p less than 0.01). Stability of sensor function was demonstrated by the absence in variation of SC and lo, calculated from the different plateaus obtained during the glucose infusions. This study provides a simple method for evaluating in vivo the function of a miniaturized sensor implanted in subcutaneous tissue.
开发了一种小型化葡萄糖传感器，它由一根完全涂有聚四氟乙烯的铂丝组成，其末端附近1毫米的一段除外，葡萄糖氧化酶固定于此。传感器对葡萄糖的体外灵敏度为2.3±0.4 nA/mM，均值±标准误（n = 23）。将这些传感器植入正常比格犬的皮下组织。进行了两次连续的葡萄糖输注（15 - 30 mg/kg/min）。传感器产生的电流用于计算灵敏度系数（SC）（nA/mM）以及无葡萄糖时的背景电流（lo）（nA）。这些参数用于测定表观皮下葡萄糖浓度。体内灵敏度低于体外灵敏度（0.5±0.1对2.2±0.2；n = 12次比较；p＜0.01）。根据葡萄糖输注期间获得的不同平台期计算得出的SC和lo无变化，证明了传感器功能的稳定性。本研究提供了一种简单的方法来体内评估植入皮下组织的小型化传感器的功能。
