Finkina E I, Balandin S V, Serebryakova M V, Potapenko N A, Tagaev A A, Ovchinnikova T V
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117997 Moscow, Russia.
Biochemistry (Mosc). 2007 Apr;72(4):430-8. doi: 10.1134/s0006297907040104.
A subfamily of eight novel lipid transfer proteins designated as Lc-LTP1-8 was found in the lentil Lens culinaris. Lc-LTP2, Lc-LTP4, Lc-LTP7, and Lc-LTP8 were purified from germinated lentil seeds, and their molecular masses (9268.7, 9282.7, 9121.5, 9135.5 daltons) and complete amino acid sequences were determined. The purified proteins consist of 92-93 amino acid residues, have four disulfide bonds, and inhibit growth of Agrobacterium tumefaciens. Total RNA was isolated from germinated lentil seeds, RT-PCR and cloning were performed, and the cDNAs of six LTPs were sequenced. Precursor 116-118-residue proteins with 24-25-residue signal peptides were found, and two of them are purified proteins Lc-LTP2 and Lc-LTP4.
在兵豆（Lens culinaris）中发现了一个由8种新型脂质转移蛋白组成的亚家族，命名为Lc-LTP1-8。从萌发的兵豆种子中纯化出了Lc-LTP2、Lc-LTP4、Lc-LTP7和Lc-LTP8，并测定了它们的分子量（分别为9268.7、9282.7、9121.5、9135.5道尔顿）和完整的氨基酸序列。纯化后的蛋白质由92-93个氨基酸残基组成，有4个二硫键，并且能够抑制根癌土壤杆菌的生长。从萌发的兵豆种子中分离出总RNA，进行逆转录-聚合酶链反应（RT-PCR）和克隆，并对6种脂质转移蛋白（LTPs）的cDNA进行测序。发现了具有24-25个氨基酸残基信号肽的116-118个氨基酸残基的前体蛋白，其中两种是纯化后的蛋白Lc-LTP2和Lc-LTP4。
