一种来自小扁豆（兵豆）种子的新型防御素。

A novel defensin from the lentil Lens culinaris seeds.

作者信息

Finkina Ekaterina I, Shramova Elena I, Tagaev Andrey A, Ovchinnikova Tatiana V

机构信息

Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str. 16/10, 117997 Moscow, Russia.

出版信息

Biochem Biophys Res Commun. 2008 Jul 11;371(4):860-5. doi: 10.1016/j.bbrc.2008.04.161. Epub 2008 May 9.

DOI:10.1016/j.bbrc.2008.04.161

PMID:18468512

Abstract

A novel 47-residue plant defensin was purified from germinated seeds of the lentil Lens culinaris by ammonium sulfate precipitation, gel filtration, chromatography, and RP-HPLC. The molecular mass (5440.41Da) and complete amino acid sequence (KTCENLSDSFKGPCIPDGNCNKHCKEKEHLLSGRCRDDFRCWCTRNC) of defensin, termed Lc-def, were determined. Lc-def has eight cysteines forming four disulfide bonds. The total RNA was isolated from lentil germinated seeds, RT-PCR and subsequent cloning were performed, and cDNA was sequenced. A 74-residue predefensin contains a putative signal peptide (27 amino acid) and a mature protein. Lc-def shows high sequence homology with legumes defensins, exhibits an activity against Aspergillus niger, but does not inhibit proteolytic enzymes.

摘要

通过硫酸铵沉淀、凝胶过滤、色谱法和反相高效液相色谱法，从扁豆（Lens culinaris）发芽种子中纯化出一种新型的47个氨基酸残基的植物防御素。确定了该防御素（命名为Lc-def）的分子量（5440.41Da）和完整氨基酸序列（KTCENLSDSFKGPCIPDGNCNKHCKEKEHLLSGRCRDDFRCWCTRNC）。Lc-def有八个半胱氨酸，形成四个二硫键。从扁豆发芽种子中分离出总RNA，进行逆转录聚合酶链反应（RT-PCR）及后续克隆，并对cDNA进行测序。一种74个氨基酸残基的前防御素包含一个推定的信号肽（27个氨基酸）和一个成熟蛋白。Lc-def与豆科植物防御素具有高度的序列同源性，对黑曲霉具有活性，但不抑制蛋白水解酶。