de Sá A R, Moreira P R, Xavier G M, Sampaio I, Kalapothakis E, Dutra W O, Gomez R S
Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Int Endod J. 2007 Jul;40(7):563-72. doi: 10.1111/j.1365-2591.2007.01272.x. Epub 2007 May 18.
To investigate in individuals with symptomatic dental abscesses the occurrence of functional polymorphisms within five genes involved with the immune response. The functional gene polymorphisms analysed were CD14 (-260 C/T), IL1B (+3954 C/T), IL6 (-174 G/C,), IL10 (-1082 G/A) and TNFA (-308 G/A).
Genomic DNA obtained from oral swabs from individuals with symptomatic dental abscesses and asymptomatic inflammatory periapical lesions, without previous exacerbation, was submitted to restriction fragment length polymorphism (RFLP) analyses to determine each individual genotype. The chi-square and principal components analysis tests were used for statistical analysis.
A significant association was observed between the occurrence of the GG genotype or the G allele expression of the polymorphic locus-174 (G/C) of the IL6 gene, and the presence of the symptomatic dental abscesses in women and in individuals < or =35 years old. The principal components analysis suggested predominance of the symptomatic dental abscesses in individuals displaying: high-producer IL6 genotype; intermediate and high-producer IL1B genotypes and low-producer TNFA genotype.
The present study suggests that genetic factors are associated with susceptibility to develop symptomatic dental abscesses.
调查有症状牙脓肿患者中涉及免疫反应的五个基因内功能多态性的发生情况。所分析的功能基因多态性为CD14（-260 C/T）、IL1B（+3954 C/T）、IL6（-174 G/C）、IL10（-1082 G/A）和TNFA（-308 G/A）。
从有症状牙脓肿和无症状炎性根尖周病变且既往无病情加重的个体的口腔拭子中获取基因组DNA，进行限制性片段长度多态性（RFLP）分析以确定每个个体的基因型。采用卡方检验和主成分分析进行统计分析。
观察到IL6基因多态性位点-174（G/C）的GG基因型或G等位基因表达的发生与女性及年龄≤35岁个体中有症状牙脓肿的存在之间存在显著关联。主成分分析表明，有症状牙脓肿在表现为以下基因型的个体中占优势：IL6高产生基因型；IL1B中产生和高产生基因型以及TNFA低产生基因型。
本研究表明遗传因素与发生有症状牙脓肿的易感性相关。
