Miri-Moghaddam Ebrahim, Farhad Mollashahi Narges, Naghibi Nava, Garme Yasaman, Bazi Ali
Cellular & Molecular Research Center & Dept. of Molecular Medicine, Birjand University of Medical Sciences, Birjand, Iran.
Genetics of Non-Communicable Disease Research Center & Dept. of Genetics, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
J Dent (Shiraz). 2018 Jun;19(2):109-117.
Genetic polymorphisms can alter immunity response against pathogens, which in turn influence individuals' susceptibility to certain infections.
Our aim was to determine the association of Arg753Gln (rs5743708) and Arg677Trp (rs12191786) polymorphisms of toll like receptor-2 gene with the two clinical forms of apical periodontitis: acute apical abscess (AAA) and asymptomatic apical periodontitis (AAP).
There were 50 patients with AAA as case group and 50 with AAP as control group. Genotyping was done using Tetra-ARMS (amplification refractory mutation system) PCR.
Heterozygous genotype of Arg677Trp polymorphism was associated with risk of AAA (OR=1.9, 95% CI: 0.7-5.5, = 0.05). Although statistically insignificant, Arg677Trp polymorphism promoted the risk of AAA in dominant model (OR=2.1, 95% CI: 0.7-5.9, > 0.05). The frequency of mutant allele (T) of Arg677Trp polymorphism was higher in AAA (14%) than AAP (7%) subjects (OR=1.7, 95% CI: 0.6-4.7). For Arg753Gln polymorphism, wild homozygous (GG) represented the dominant genotype in both cases (96%) and controls (100%). Variant allele (A) of Arg753Gln polymorphism was identified in 2% of AAA, while no individual represented with this allele in AAP subjects. Individuals with Arg753Gln; Arg677Trp (GG; TC) combination showed an elevated risk of AAA (OR=1.6, 95% CI: 0.5- 4.2, > 0.05).
Arg677Trp polymorphism of TLR-2 rendered a higher risk for the development of abscesses in apical periodontitis. It is recommended to explore role of this polymorphism in other populations.
基因多态性可改变针对病原体的免疫反应，进而影响个体对某些感染的易感性。
我们的目的是确定Toll样受体2基因的Arg753Gln（rs5743708）和Arg677Trp（rs12191786）多态性与根尖周炎的两种临床形式：急性根尖脓肿（AAA）和无症状根尖周炎（AAP）之间的关联。
将50例AAA患者作为病例组，50例AAP患者作为对照组。采用四引物扩增不应突变系统（Tetra-ARMS）PCR进行基因分型。
Arg677Trp多态性的杂合基因型与AAA风险相关（OR = 1.9，95%CI：0.7 - 5.5，P = 0.05）。虽然在统计学上无显著意义，但Arg677Trp多态性在显性模型中增加了AAA风险（OR = 2.1，95%CI：0.7 - 5.9，P>0.05）。AAA患者中Arg677Trp多态性的突变等位基因（T）频率（14%）高于AAP患者（7%）（OR = 1.7，95%CI：0.6 - 4.7）。对于Arg753Gln多态性，野生纯合子（GG）在病例组（96%）和对照组（100%）中均为主要基因型。在2%的AAA患者中鉴定出Arg753Gln多态性的变异等位基因（A），而AAP患者中无个体携带该等位基因。具有Arg753Gln；Arg677Trp（GG；TC）组合的个体显示出较高的AAA风险（OR = 1.6，95%CI：0.5 - 4.2，P>0.05）。
TLR-2的Arg677Trp多态性使根尖周炎发生脓肿的风险更高。建议在其他人群中探索这种多态性的作用。
