Fair T, Carter F, Park S, Evans A C O, Lonergan P
School of Agriculture, Food Science and Veterinary Medicine, University College Dublin, Agriculture & Food Science Building, Belfield, Dublin 4, Ireland.
Theriogenology. 2007 Sep 1;68 Suppl 1:S91-7. doi: 10.1016/j.theriogenology.2007.04.018. Epub 2007 May 18.
It is well known that factors such as nutrition, hormonal alterations and environmental compounds can have a profound effect on oocyte quality and subsequently embryo development. Global mRNA expression analysis of immature and mature bovine oocytes was carried out to identify key pathways associated with oocyte meiotic maturation and developmental potential. We have carried out a global mRNA gene expression analysis of bovine oocytes pre- and post-resumption of meiotic maturation using the Affymetrix GeneChip Bovine Genome Array. Approximately 54% of the probe sets representing 23,000 transcripts were detected in bovine oocytes. Of which, 821 transcripts were differentially expressed (>or=2-fold) between the two groups (p<0.05), corresponding to 209 upregulated and 612 down regulated transcripts in the in vitro matured oocytes compared to their immature counterparts. In addition, transcripts uniquely detected in germinal vesicle stage or metaphase II were identified. The gene expression data was classified according to gene ontology; in terms of biological processing, the majority of these genes were associated with regulation activities, including the regulation of: MAPK activity, translation initiation and transcription. Our findings are in agreement with similar data from both mouse and human oocytes. Taken together this data provides a molecular transcriptome blueprint of immature and mature mammalian oocytes. This resource will be invaluable to our long-term objective which is to target identified pathways to elucidate the sensitivity of key regulatory genes and checkpoints of oocyte maturation to internal and external environmental influences.
众所周知，营养、激素变化和环境化合物等因素会对卵母细胞质量以及随后的胚胎发育产生深远影响。对未成熟和成熟的牛卵母细胞进行了全基因组mRNA表达分析，以确定与卵母细胞减数分裂成熟和发育潜力相关的关键途径。我们使用Affymetrix GeneChip牛基因组阵列对减数分裂成熟恢复前后的牛卵母细胞进行了全基因组mRNA基因表达分析。在牛卵母细胞中检测到了代表23,000个转录本的约54%的探针集。其中，两组之间有821个转录本差异表达（≥2倍）（p<0.05），与未成熟卵母细胞相比，体外成熟卵母细胞中有209个转录本上调，612个转录本下调。此外，还鉴定了在生发泡期或中期II中独特检测到的转录本。基因表达数据根据基因本体进行分类；在生物学过程方面，这些基因中的大多数与调控活动相关，包括：MAPK活性、翻译起始和转录的调控。我们的发现与来自小鼠和人类卵母细胞的类似数据一致。综合这些数据提供了未成熟和成熟哺乳动物卵母细胞的分子转录组蓝图。这一资源对于我们的长期目标将是非常宝贵的，我们的长期目标是针对已确定的途径来阐明关键调控基因的敏感性以及卵母细胞成熟对内部和外部环境影响的检查点。
