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通过基质辅助激光解吸/电离对非共价蛋白质复合物的研究。

A study of noncovalent protein complexes by matrix-assisted laser desorption/ionization.

作者信息

Song Fenhong

机构信息

Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville, Maryland, USA.

出版信息

J Am Soc Mass Spectrom. 2007 Jul;18(7):1286-90. doi: 10.1016/j.jasms.2007.03.026. Epub 2007 Apr 6.

DOI:10.1016/j.jasms.2007.03.026
PMID:17512214
Abstract

A sample preparation method has been developed for detection of noncovalent protein-protein complexes by MALDI in this work. An aqueous solution of the matrix at pH 7 allows the reproducible detection of a protein dimer, a protein tetramer, and a heterodimer. The signals are stable under long irradiation and can be detected at wide ranges of concentrations and with different laser intensities.

摘要

在本研究中，已开发出一种用于通过基质辅助激光解吸电离（MALDI）检测非共价蛋白质 - 蛋白质复合物的样品制备方法。pH值为7的基质水溶液能够实现对蛋白质二聚体、蛋白质四聚体和异源二聚体的可重复检测。这些信号在长时间照射下稳定，并且在广泛的浓度范围和不同激光强度下均可检测到。

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