含CpG寡核苷酸和明矾的疫苗配方。
Formulation of vaccines containing CpG oligonucleotides and alum.
作者信息
Aebig Joan A, Mullen Gregory E D, Dobrescu Gelu, Rausch Kelly, Lambert Lynn, Ajose-Popoola Olubunmi, Long Carole A, Saul Allan, Miles Aaron P
机构信息
Malaria Vaccine Development Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 5640 Fishers Lane, Rockville, MD, 20852 USA.
出版信息
J Immunol Methods. 2007 Jun 30;323(2):139-46. doi: 10.1016/j.jim.2007.04.003. Epub 2007 May 11.
Abstract
CpG oligodeoxynucleotides are potent immunostimulants. For parenterally delivered alum-based vaccines, the immunostimulatory effect of CpG depends on the association of the CpG and antigen to the alum. We describe effects of buffer components on the binding of CPG 7909 to aluminum hydroxide (Alhydrogel), assays for measuring binding of CPG 7909 to alum and CPG 7909 induced dissociation of antigen from the alum. Free CPG 7909 is a potent inducer of IP-10 in mice. However the lack of IP-10 production from formulations containing bound CPG 7909 suggested that CPG 7909 does not rapidly dissociate from the alum after injection. It also suggests that IP-10 assays are not a good basis for potency assays for alum-based vaccines containing CPG 7909.
摘要
CpG 寡脱氧核苷酸是强效免疫刺激剂。对于通过肠胃外途径递送的铝基疫苗，CpG 的免疫刺激作用取决于 CpG 与抗原和铝盐的结合。我们描述了缓冲液成分对 CPG 7909 与氢氧化铝（铝佐剂）结合的影响、测量 CPG 7909 与铝盐结合的测定方法以及 CPG 7909 诱导抗原从铝盐解离的情况。游离的 CPG 7909 是小鼠中 IP-10 的强效诱导剂。然而，含有结合态 CPG 7909 的制剂未能产生 IP-10，这表明 CPG 7909 在注射后不会迅速从铝盐中解离。这也表明 IP-10 测定法并非含有 CPG 7909 的铝基疫苗效力测定的良好依据。
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