Yang Zhou, Zhang Xiaohong, Gang Haiju, Li Xiaojun, Li Zumao, Wang Tao, Han Juan, Luo Ting, Wen Fuqiang, Wu Xiaoting
Department of General Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan, PR China.
Biochem Biophys Res Commun. 2007 Jul 6;358(3):925-30. doi: 10.1016/j.bbrc.2007.05.023. Epub 2007 May 15.
Twist, a newly found EMT-inducer, has been reported to be up-regulated in those of diffuse-type gastric carcinomas with high N-cadherin level. We show here MKN45, a cell line derived from undifferentiated carcinomas cells, expresses high levels of Twist. Down-regulation of Twist, using an antisense Twist vector in MKN45 cells, inhibits cell migration and invasion, companied with a morphologic changes associated with MET. Suppression of Twist also decreases the expressions of N-cadherin and fibronectin, but not of E-cadherin in MKN45. In contrast, overexpression of Twist in MKN28, a cell line derived from moderate differentiated carcinomas, results in up-regulation of N-cadherin and fibronectin, companied with down-regulation of E-cadherin. Taken together, our results suggest that Twist regulates cell motility and invasion in gastric cancer cell lines, probably through the N-cadherin and fibronectin production.
Twist是一种新发现的上皮-间质转化诱导因子，据报道在N-钙黏蛋白水平高的弥漫型胃癌中表达上调。我们在此表明，源自未分化癌细胞的细胞系MKN45表达高水平的Twist。在MKN45细胞中使用反义Twist载体下调Twist，可抑制细胞迁移和侵袭，并伴有与上皮-间质转化相关的形态学变化。抑制Twist还可降低MKN45中N-钙黏蛋白和纤连蛋白的表达，但不影响E-钙黏蛋白的表达。相反，在源自中度分化癌的细胞系MKN28中过表达Twist，会导致N-钙黏蛋白和纤连蛋白上调，同时E-钙黏蛋白下调。综上所述，我们的结果表明，Twist可能通过产生N-钙黏蛋白和纤连蛋白来调节胃癌细胞系中的细胞运动和侵袭。
