Yang Zhou, Li Xiao-Jun, Luo Ting, Wu Xiao-Ting
Department of General Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2011 Sep;42(5):625-9.
To determine the level of TWIST expression and its clinical significance in gastric adenocarcinoma.
Tumor specimens resected from 43 patients with gastric carcinoma were obtained, and 12 normal gastric tissues were used as normal control. The expression level of TWIST was detected by immunohistochemical staining. Furthermore, The distinctive expression of TWIST in MKN45 and MKN28 gastric cell lines was studied by western blot analyze, and the subcellular localization of TWIST in MKN45 cells was examined by the method of confocal microscopy.
The positive expression rate of TWIST in gastric cancerous tissue was 58.14%, which was significantly higher than that in normal tissue (P < 0.01). The expression rate in the patients with metastasis was also significantly higher than that in those with locally advanced disease (P < 0.01). In MKN45 cells, TWIST was observed locating in cytoplasm and nucleus, predominately at perinuclear area in the cytoplasm. Western blot demonstrated an expected band in both cytoplasmic and nuclear extracts from MKN45 cells. In addition, MKN45, a cell line derived from undierentiated carcinomas cells, expressed high level of TWIST. While MKN28, a cell line derived from moderate dierentiated carcinomas, showed a lower expression of TWIST.
Overexpression of TWIST may correlate to tumor invasion in the gastric adenocarcinoma.
检测TWIST在胃腺癌中的表达水平并探讨其临床意义。
收集43例胃癌患者手术切除的肿瘤标本，取12例正常胃组织作为对照。采用免疫组化染色检测TWIST的表达水平。此外，通过蛋白质印迹分析研究TWIST在MKN45和MKN28胃癌细胞系中的特异性表达，并采用共聚焦显微镜方法检测TWIST在MKN45细胞中的亚细胞定位。
TWIST在胃癌组织中的阳性表达率为58.14%，显著高于正常组织（P<0.01）。转移患者中的表达率也显著高于局部进展期患者（P<0.01）。在MKN45细胞中，TWIST定位于细胞质和细胞核，主要位于细胞质中的核周区域。蛋白质印迹显示在MKN45细胞的细胞质和细胞核提取物中均出现预期条带。此外，来源于未分化癌细胞的MKN45细胞系表达高水平的TWIST。而来源于中度分化癌的MKN28细胞系TWIST表达较低。
TWIST的过表达可能与胃腺癌的肿瘤侵袭相关。
