人胰腺导管上皮细胞三维培养体系的建立。
Establishment of three-dimensional cultures of human pancreatic duct epithelial cells.
作者信息
Gutierrez-Barrera Angelica M, Menter David G, Abbruzzese James L, Reddy Shrikanth A G
机构信息
Department of Gastrointestinal Medical Oncology, The University of Texas M.D. Anderson Cancer Center, Unit 426, 1515 Holcombe Boulevard, Houston, TX 77030, USA.
出版信息
Biochem Biophys Res Commun. 2007 Jul 6;358(3):698-703. doi: 10.1016/j.bbrc.2007.04.166. Epub 2007 May 4.
Three-dimensional (3D) cultures of epithelial cells offer singular advantages for studies of morphogenesis or the role of cancer genes in oncogenesis. In this study, as part of establishing a 3D culture system of pancreatic duct epithelial cells, we compared human pancreatic duct epithelial cells (HPDE-E6E7) with pancreatic cancer cell lines. Our results show, that in contrast to cancer cells, HPDE-E6E7 organized into spheroids with what appeared to be apical and basal membranes and a luminal space. Immunostaining experiments indicated that protein kinase Akt was phosphorylated (Ser473) and CTMP, a negative Akt regulator, was expressed in both HPDE-E6E7 and cancer cells. However, a nuclear pool of CTMP was detectable in HPDE-E6E7 cells that showed a dynamic concentrated expression pattern, a feature that further distinguished HPDE-E637 cells from cancer cells. Collectively, these data suggest that 3D cultures of HPDE-E6E7 cells are useful for investigating signaling and morphological abnormalities in pancreatic cancer cells.
上皮细胞的三维（3D）培养为形态发生研究或癌症基因在肿瘤发生中的作用提供了独特优势。在本研究中，作为建立胰腺导管上皮细胞3D培养系统的一部分，我们将人胰腺导管上皮细胞（HPDE-E6E7）与胰腺癌细胞系进行了比较。我们的结果表明，与癌细胞不同，HPDE-E6E7形成了具有顶端和基底膜以及管腔空间的球体。免疫染色实验表明，蛋白激酶Akt被磷酸化（Ser473），并且负性Akt调节因子CTMP在HPDE-E6E7和癌细胞中均有表达。然而，在HPDE-E6E7细胞中可检测到CTMP的核池，其呈现动态浓缩表达模式，这一特征进一步将HPDE-E637细胞与癌细胞区分开来。总体而言，这些数据表明HPDE-E6E7细胞的3D培养对于研究胰腺癌细胞中的信号传导和形态异常是有用的。
Zotero 插件