MYCN反义DNA寡聚体对人神经母细胞瘤细胞系增殖率的降低及分化的诱导作用
Decrease of proliferation rate and induction of differentiation by a MYCN antisense DNA oligomer in a human neuroblastoma cell line.
作者信息
Negroni A, Scarpa S, Romeo A, Ferrari S, Modesti A, Raschellà G
机构信息
Division of Physics and Biochemical Sciences, Ente per le nuove technologie per l'energia e l'ambiente (ENEA), Rome, Italy.
出版信息
Cell Growth Differ. 1991 Oct;2(10):511-8.
The effects of an antisense oligodeoxynucleotide to codons 2-7 of the oncogene MYCN on the human neuroblastoma cell line LAN-5 were studied. Treated cells showed a decreased MYCN protein expression and synthesis by immunoperoxidase staining and immunoprecipitation. At the same time, the replication rate was inhibited, and the phenotype was modified toward a more differentiated type. Our data suggest the involvement of oncogene MYCN in both proliferative and differentiative processes.
研究了一种针对癌基因MYCN密码子2 - 7的反义寡脱氧核苷酸对人神经母细胞瘤细胞系LAN - 5的影响。通过免疫过氧化物酶染色和免疫沉淀法检测发现，经处理的细胞中MYCN蛋白表达和合成减少。同时，细胞复制速率受到抑制，细胞表型向更分化的类型转变。我们的数据表明癌基因MYCN参与了增殖和分化过程。
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