Dafar Amal, Siarov Angelica, Mostaghimi Yasaman, Robledo-Sierra Jairo, De Lara Shahin, Giglio Daniel, Kjeller Göran, Braz-Silva Paulo Henrique, Öhman Jenny, Hasséus Bengt
Department of Oral Medicine and Pathology, Institute of Odontology, The Sahlgrenska Academy, University of Gothenburg, Gothenburg 40530, Sweden.
Department of Oral and Maxillofacial Surgery, King Fahad General Hospital, Jeddah 23325, Saudi Arabia.
Int J Dent. 2022 Mar 22;2022:5430309. doi: 10.1155/2022/5430309. eCollection 2022.
Although oral lichen planus (OLP) and oral leukoplakia (LPL) have different pathogenetic profiles, both may involve chronic inflammation. The aim of this observational study was to evaluate the inflammatory cell profiles of OLP and LPL. The inflammatory cell infiltrates in patients with OLP and LPL were analyzed for the presence of Langerhans cells (LCs; CD1a), T cells (CD3), and B cells (CD20), as well as for the proliferation marker Ki-67. Biopsied specimens from patients with OLP ( = 14) and LPL without dysplasia ( = 13) were immunohistochemically stained with antibodies directed against CD1a, CD3, CD20, and Ki-67, followed by quantitative analyses. A significant increase in the number of CD3 cells and CD20 cells was found in the submucosa of OLP, as compared to LPL ( < 0.01). Likewise, the number of CD3 cells was significantly higher in the epithelium of OLP than of LPL ( < 0.05). No differences were found in the expression of Ki-67 and the number of CD1a cells between the two groups. Although an immune response is elicited in both conditions, there are differences at the cellular level between OLP and LPL. A more robust immune activation involving T cells and B cells is seen in OLP. The role of B cells in OLP needs to be further elucidated. Although the number of B cells in LPL is low, their role in the inflammatory response cannot be ruled out.
尽管口腔扁平苔藓（OLP）和口腔白斑（LPL）具有不同的发病机制，但两者都可能涉及慢性炎症。本观察性研究的目的是评估OLP和LPL的炎症细胞谱。分析OLP和LPL患者炎症细胞浸润中朗格汉斯细胞（LCs；CD1a）、T细胞（CD3）和B细胞（CD20）的存在情况，以及增殖标志物Ki-67。对OLP患者（n = 14）和无发育异常的LPL患者（n = 13）的活检标本进行免疫组织化学染色，使用针对CD1a、CD3、CD20和Ki-67的抗体，随后进行定量分析。与LPL相比，OLP黏膜下层中CD3细胞和CD20细胞数量显著增加（P < 0.01）。同样，OLP上皮中CD3细胞数量显著高于LPL（P < 0.05）。两组之间Ki-67的表达和CD1a细胞数量未发现差异。尽管在这两种情况下都会引发免疫反应，但OLP和LPL在细胞水平上存在差异。在OLP中可见涉及T细胞和B细胞的更强有力的免疫激活。B细胞在OLP中的作用需要进一步阐明。尽管LPL中B细胞数量较少，但其在炎症反应中的作用不能排除。
