雄激素对小鼠睑板腺中胆固醇生成酶mRNA水平的影响。
Androgen influence on cholesterogenic enzyme mRNA levels in the mouse meibomian gland.
作者信息
Schirra Frank, Richards Stephen M, Sullivan David A
机构信息
Harvard Medical School, Schepens Eye Research Institute, 20 Staniford Street, Boston, MA 02114, USA.
出版信息
Curr Eye Res. 2007 May;32(5):393-8. doi: 10.1080/02713680701316674.
PURPOSE
We tested our hypothesis that testosterone increases the meibomian gland gene expression of numerous enzymes in the cholesterol biosynthetic pathway.
METHODS
Meibomian glands were obtained from castrated mice treated with vehicle or testosterone for 2 weeks. Tissues were processed for the analysis of selected mRNAs by real-time PCR.
RESULTS
Our research demonstrates that testosterone stimulates a significant increase in the mRNA levels of mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylase, isopentenyl pyrophosphate isomerase, geranylgeranyl pyrophosphate synthase, squalene epoxidase, lanosterol synthase, lanosterol demethylase, and Delta 7-sterol reductase.
CONCLUSIONS
Our findings indicate that androgens may promote cholesterol biosynthesis in the meibomian gland.
目的
我们检验了以下假设，即睾酮会增加睑板腺中胆固醇生物合成途径中多种酶的基因表达。
方法
从接受载体或睾酮处理2周的去势小鼠获取睑板腺。通过实时PCR对组织进行处理以分析选定的mRNA。
结果
我们的研究表明，睾酮可显著刺激甲羟戊酸激酶、磷酸甲羟戊酸激酶、甲羟戊酸焦磷酸脱羧酶、异戊烯基焦磷酸异构酶、香叶基香叶基焦磷酸合酶、角鲨烯环氧酶、羊毛甾醇合酶、羊毛甾醇脱甲基酶和Δ7-甾醇还原酶的mRNA水平升高。
结论
我们的研究结果表明，雄激素可能促进睑板腺中的胆固醇生物合成。
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