Caillou Samuel, Boonaert Christophe J P, Dewez Jean-Luc, Rouxhet Paul G
Unité de chimie des interfaces, Université catholique de Louvain, Croix du Sud 2/18, B-1348 Louvain-la-Neuve, Belgium.
J Biomed Mater Res B Appl Biomater. 2008 Jan;84(1):240-8. doi: 10.1002/jbm.b.30866.
The cleaning of cellulosic hemodialysis membrane Cuprophan and model materials (glass; polystyrene and polypropylene, as such and surface-oxidized), conditioned by adsorption of blood plasma proteins (HSA, fibrinogen, IgG) was investigated in vitro. Sodium hypochlorite (NaClO) and Renalin, a product containing hydrogen peroxide and peroxyacetic acid, were used as cleaning reagents. X-ray photoelectron spectroscopy and the use of radiolabeled fibrinogen demonstrated the presence of varying amounts of a polypeptidic residue, with sulfur brought to a high oxidation stage (sulfonate-like). The trends were the same for the three proteins regarding the effectiveness of the oxidizer and the influence of the surface properties. NaClO was much more effective than Renalin to remove the adsorbed proteins. The proteins adsorbed on Cuprophan were more sensitive to the oxidizers, when compared with proteins adsorbed on other materials. This may be due to both the lower protein-surface affinity, as indicated by radiochemical measurements, and the sensitivity of the material itself to the oxidizer, as revealed by weight loss measurements. These results support the attribution of hemocompatibility improvement after regeneration of Cuprophan with Renalin to the masking of the activating surface by a residue from previously adsorbed proteins.
体外研究了经血浆蛋白（人血清白蛋白、纤维蛋白原、免疫球蛋白）吸附处理的铜仿纤维素血液透析膜及模型材料（玻璃、聚苯乙烯和聚丙烯，原样及表面氧化的）的清洁情况。使用次氯酸钠（NaClO）和含有过氧化氢和过氧乙酸的产品Renalin作为清洁试剂。X射线光电子能谱和放射性标记纤维蛋白原的使用表明存在不同量的多肽残基，其中硫处于高氧化态（类似磺酸盐）。就氧化剂的有效性和表面性质的影响而言，三种蛋白质的趋势相同。NaClO在去除吸附蛋白方面比Renalin有效得多。与吸附在其他材料上的蛋白质相比，吸附在铜仿膜上的蛋白质对氧化剂更敏感。这可能是由于放射化学测量表明的较低的蛋白质-表面亲和力以及失重测量显示的材料本身对氧化剂的敏感性。这些结果支持了用Renalin再生后铜仿膜血液相容性改善归因于先前吸附蛋白质的残基对活化表面的掩盖这一观点。
