Zhang Ying-hua, Shi Xun-li, Liu Ya-lan, Liu Cui-ge, Wei Yong-ju
College of Chemistry, Hebei Normal University, Shijiazhuang 050016, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2007 Feb;27(2):342-5.
Fluorescence spectra of chang shan (Dichroa febrifuga Lour) aqueous extraction were studied. In the three-dimensional fluorescence contour spectrum, three fluorescence peaks of quinazoline alkaloids, which are the active components of chang shan, were observed. The excitation wavelengths of the peaks were 235, 270 and 320 nm, respectively, and the emission wavelength of all the peaks was 430 nm. Three-dimensional fluorescence contour spectrum is the very image of fingerprint, suitable for qualitative identification of traditional Chinese medicine. In the range of pH 3 to pH 6, the fluorescence spectrum of chang shan aqueous extractions changes with the variation in pH value. The reason for this spectral change might be the protonation of N-1 in quinazolone ring of beta-dichroine (febrifugine) molecule. There is an excellent linear relationship between the fluorescence intensity and the concentration of chang shan under nearly neutral conditions, thereby a quantitative method for the determination of quinazoline alkaloids may be established.
对常山（Dichroa febrifuga Lour）水提取物的荧光光谱进行了研究。在三维荧光等高线光谱中，观察到常山活性成分喹唑啉生物碱的三个荧光峰。这些峰的激发波长分别为235、270和320nm，所有峰的发射波长均为430nm。三维荧光等高线光谱是指纹图谱的直观图像，适用于中药的定性鉴别。在pH值3至6范围内，常山水提取物的荧光光谱随pH值变化而变化。这种光谱变化的原因可能是β-常山碱（常山碱）分子喹唑酮环中N-1的质子化。在近中性条件下，荧光强度与常山浓度之间存在良好的线性关系，从而可建立一种测定喹唑啉生物碱的定量方法。
