Jin Dan, Zhang Yu-jun, Li Guo-gang, Xiao Xue, Wang Zhi-gang, Yin Gao-fang, Liu Wen-qing
Key Laboratory of Environmental Optics & Technology, Anhui Institute of Optics and Fine Mechanics, The Chinese Academy of Sciences, Hefei 230031, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2009 May;29(5):1319-22.
According to the high fluorescence quantum yields of polycyclic aromatic hydrocarbons (PAHs), the fluorescence spectra of phenanthrene were investigated by three dimensional fluorescence excitation-emission matrix (3DEEM). The results show that the three-dimensional fluorescence spectra of phenanthrene in aqueous solution mainly have two fluorescence peaks. On the basis of three-dimensional fluorescence spectrometry analysis of phenanthrene, the excitation wavelength of 255 nm and emission wavelength of 273 nm were chosen for the quantitative analysis of phenanthrene. The linear range for the determination of phenanthrene was 5.0-250.0 mg x mL(-1), its detection limit was 3. 88 ng x mL(-1), and its relative standard deviation was 4.23% (n=5). It was a good precision. It has been tested satisfactorily for the determination of artificial sample in tap water. The recoveries are in the range of 90%-105%. The method provided basis for the rapid monitoring of trace PAHs in water.
基于多环芳烃（PAHs）较高的荧光量子产率，采用三维荧光激发-发射矩阵（3DEEM）对菲的荧光光谱进行了研究。结果表明，菲在水溶液中的三维荧光光谱主要有两个荧光峰。在对菲进行三维荧光光谱分析的基础上，选择激发波长255 nm和发射波长273 nm对菲进行定量分析。菲的测定线性范围为5.0 - 250.0 mg·mL⁻¹，检出限为3.88 ng·mL⁻¹，相对标准偏差为4.23%（n = 5），精密度良好。该方法用于自来水中人工样品的测定，结果令人满意，回收率在90% - 105%范围内。该方法为水中痕量多环芳烃的快速监测提供了依据。
