Bertho P, Moreau P, Morré D J, Cassagne C
Institut de Biochimie et Neurochimie du CNRS, Université de Bordeaux II, France.
Biochim Biophys Acta. 1991 Nov 18;1070(1):127-34. doi: 10.1016/0005-2736(91)90154-z.
Delivery of newly synthesized fatty acids and lipids to the plasma membrane in leek seedlings via the endoplasmic reticulum (ER)-Golgi apparatus pathway is primarily by bulk transport (without sorting). However, pulse-chase experiments revealed kinetics of transport of lipids with VLCFA (very long chain fatty acids having more than 18 carbon atoms) in favor of a preferential transfer of these molecules to the plasma membrane. Use of monensin showed the accumulation of lipids in the Golgi apparatus and a related decrease of the amount of lipids transported to the plasma membrane. Lipid and fatty acid analyses revealed that transport of VLCFA-containing phospholipids was most strongly inhibited by the monensin block. These results taken together with an inability of the plasma membrane to synthesize VLCFA support a role for the Golgi apparatus in VLCFA delivery to the plasma membrane and leads to the hypothesis of a sorting function as well, based on fatty acyl chain length.
在韭菜幼苗中，新合成的脂肪酸和脂质通过内质网（ER）-高尔基体途径输送到质膜主要是通过批量运输（无分选）。然而，脉冲追踪实验揭示了具有超长链脂肪酸（VLCFA，碳原子数超过18个的非常长链脂肪酸）的脂质的运输动力学，有利于这些分子优先转移到质膜。莫能菌素的使用显示脂质在高尔基体中积累，以及运输到质膜的脂质量相应减少。脂质和脂肪酸分析表明，含VLCFA的磷脂的运输受到莫能菌素阻断的抑制作用最强。这些结果与质膜无法合成VLCFA的情况相结合，支持了高尔基体在将VLCFA输送到质膜中的作用，并且基于脂肪酰链长度也引出了分选功能的假设。
