来自韭菜细胞内质网的富含磷脂酰丝氨酸囊泡的ATP依赖性形成。
ATP-Dependent formation of phosphatidylserine-rich vesicles from the endoplasmic reticulum of leek cells.
作者信息
Sturbois-Balcerzak B, Vincent P, Maneta-Peyret L, Duvert M, Satiat-Jeunemaitre B, Cassagne C, Moreau P
机构信息
Laboratoire de Biogenese Membranaire, Unite Mixte de Recherche-5544-Centre National de la Recherche Scientifique (CNRS) (B.S.-B., P.V., L.M.-P., C.C., P.M.).
出版信息
Plant Physiol. 1999 May;120(1):245-56. doi: 10.1104/pp.120.1.245.
Leek (Allium porrum) plasma membrane is enriched in phosphatidylserine (PS) by the vesicular pathway, in a way similar to that already observed in animal cells (B. Sturbois-Balcerzak, D.J. Morre, O. Loreau, J.P. Noel, P. Moreau, C. Cassagne [1995] Plant Physiol Biochem 33: 625-637). In this paper we document the formation of PS-rich small vesicles from leek endoplasmic reticulum (ER) membranes upon addition of ATP and other factors. The omission of ATP or its replacement by ATPgamma-S prevents vesicle formation. These vesicles correspond to small structures (70-80 nm) and their phospholipid composition, characterized by a PS enrichment, is compatible with a role in PS transport. Moreover, the PS enrichment over phosphatidylinositol in the ER-derived vesicles is the first example, to our knowledge, of phospholipid sorting from the ER to ER-derived vesicles in plant cells.
通过囊泡途径，韭菜（葱属植物）的质膜富含磷脂酰丝氨酸（PS），其方式类似于在动物细胞中已观察到的方式（B. 斯图尔博伊斯 - 巴尔采扎克、D.J. 莫雷、O. 洛罗、J.P. 诺埃尔、P. 莫罗、C. 卡萨涅 [1995]《植物生理学与生物化学》33: 625 - 637）。在本文中，我们记录了在添加ATP和其他因子后，韭菜内质网（ER）膜形成富含PS的小囊泡的过程。省略ATP或用ATPγ - S替代它会阻止囊泡形成。这些囊泡对应于小结构（70 - 80纳米），其磷脂组成以富含PS为特征，这与在PS转运中的作用相符。此外，据我们所知，内质网衍生囊泡中PS相对于磷脂酰肌醇的富集是植物细胞中从内质网到内质网衍生囊泡进行磷脂分选的首个例子。
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