Chudakov Dmitriy M, Lukyanov Sergey, Lukyanov Konstantin A
Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Biotechniques. 2007 May;42(5):553, 555, 557 passim. doi: 10.2144/000112470.
Photoactivatable fluorescent proteins are capable of dramatic changes in fluorescent properties in response to specific light irradiation. For example, they can be converted from cyan to green, or from green to red, or from nonfluorescent to a brightly fluorescent state. Several types of such proteins were developed recently, and some of them are already becoming popular tools to study protein mobility. Here we provide detailed recommendations on application of the monomeric green-to-red photoconvertible fluorescent protein Dendra2 for protein tracking in living cultured cells.
光激活荧光蛋白能够在特定光照射下发生荧光特性的显著变化。例如，它们可以从青色转变为绿色，或从绿色转变为红色，或从非荧光状态转变为强荧光状态。最近开发了几种这类蛋白，其中一些已成为研究蛋白质流动性的常用工具。在此，我们提供了关于单体绿色到红色光转换荧光蛋白Dendra2在活培养细胞中进行蛋白质追踪应用的详细建议。
