Chudakov Dmitriy M, Chepurnykh Tatyana V, Belousov Vsevolod V, Lukyanov Sergey, Lukyanov Konstantin A
Institute of Bioorganic Chemistry RAS, Miklukho-Maklaya 16/10, Moscow 117997, Russia Evrogen JSC, Miklukho-Maklaya 16/10, Moscow 117997, Russia.
Traffic. 2006 Oct;7(10):1304-10. doi: 10.1111/j.1600-0854.2006.00468.x. Epub 2006 Aug 2.
Photoactivatable fluorescent proteins opened principally novel possibilities to study proteins' movement pathways. In particular, reversibly photoactivatable proteins enable multiple tracking experiments in a long-drawn work with a single cell. Here we report 'protein rivers tracking' technique based on repeated identical rounds of photoactivation and subsequent images averaging, which results in dramatic increase of imaging resolution for fast protein movement events.
光激活荧光蛋白为研究蛋白质的移动路径带来了全新的可能性。特别是，可逆光激活蛋白能够在对单个细胞进行的长期研究中开展多次追踪实验。在此，我们报告一种“蛋白质河流追踪”技术，该技术基于重复相同轮次的光激活以及随后的图像平均化处理，这使得快速蛋白质移动事件的成像分辨率显著提高。
