Stefanutti Giorgio, Pierro Agostino, Smith Virpi V, Klein Nigel J, Eaton Simon
Units of Paediatric Surgery, Institute of Child Health, London, WC1N 1EH, United Kingdom.
Pediatr Res. 2007 Jul;62(1):43-8. doi: 10.1203/PDR.0b013e31806790c0.
Free radicals are important in development of intestinal ischemia-reperfusion (I/R) injury, leading to intestinal and pulmonary damage. We evaluated the effects of peroxynitrite decomposition catalyst FeTMPyP in infant intestinal I/R. Suckling rats underwent 40 min intestinal ischemia + 90 min reperfusion. At reperfusion, animals received saline or FeTMPyP. Groups were (n = 11 per group): 1) control+saline; 2) I/R+saline; 3) I/R+FeTMPyP. Increased histologic injury and ICAM-1 expression were observed in ileum of both I/R+saline and I/R+FeTMPyP rats, but P-selectin expression was increased in I/R+saline animals only versus controls. Myeloperoxidase (neutrophil infiltration marker) was increased in ileum and lungs of I/R+saline rats, but FeTMPyP prevented this in the ileum. I/R+saline animals showed higher malondialdehyde (lipid peroxidation marker) in ileum and lungs versus both control+saline and I/R+FeTMPyP rats. Glutathione was decreased in all I/R animals, but oxidized and total glutathione were higher in I/R+FeTMPyP than the I/R+saline group. Nitrate+nitrite concentration (systemic nitric oxide production) was elevated in I/R+saline but not in I/R+FeTMPyP animals. FeTMPyP provides limited protection against intestinal I/R in neonatal rats by reducing ileal P-selectin expression, systemic nitric oxide production, neutrophil infiltration in ileum and lipid peroxidation in both lungs and ileum; and preserving intestinal antioxidant capacity.
自由基在肠道缺血再灌注（I/R）损伤的发展过程中起着重要作用，会导致肠道和肺部损伤。我们评估了过氧亚硝酸盐分解催化剂FeTMPyP对幼鼠肠道I/R的影响。乳鼠经历40分钟的肠道缺血 + 90分钟的再灌注。在再灌注时，动物接受生理盐水或FeTMPyP。分组如下（每组n = 11）：1）对照组 + 生理盐水；2）I/R + 生理盐水；3）I/R + FeTMPyP。在I/R + 生理盐水组和I/R + FeTMPyP组的大鼠回肠中均观察到组织学损伤增加和ICAM - 1表达增加，但与对照组相比，仅I/R + 生理盐水组动物的P - 选择素表达增加。髓过氧化物酶（中性粒细胞浸润标志物）在I/R + 生理盐水组大鼠的回肠和肺中增加，但FeTMPyP可预防回肠中的这种情况。与对照组 + 生理盐水组和I/R + FeTMPyP组大鼠相比，I/R + 生理盐水组动物的回肠和肺中的丙二醛（脂质过氧化标志物）更高。所有I/R组动物的谷胱甘肽均减少，但I/R + FeTMPyP组的氧化型和总谷胱甘肽高于I/R + 生理盐水组。I/R + 生理盐水组的硝酸盐 + 亚硝酸盐浓度（全身一氧化氮产生）升高，但I/R + FeTMPyP组动物未升高。FeTMPyP通过降低回肠P - 选择素表达、全身一氧化氮产生、回肠中性粒细胞浸润以及肺和回肠中的脂质过氧化；并保留肠道抗氧化能力，为新生大鼠的肠道I/R提供有限的保护。
