Talián Gábor C, Komlósi Katalin, Decsi Tamás, Koletzko Berthold, Melegh Béla
Department of Medical Genetics and Child Development, University of Pécs, Pécs, Hungary, H-7624.
Pediatr Res. 2007 Jul;62(1):88-92. doi: 10.1203/PDR.0b013e3180676cca.
We studied plasma concentrations of free carnitine and 30 carnitine esters by electron spray ionization (ESI) tandem mass spectrometry in 37 pregnant women at the 20th and 30th weeks of gestation and at delivery, and in their neonates at birth, and in 22 age-matched nonpregnant women. The plasma levels of acetylcarnitine and carnitine esters with more than five carbons were significantly higher, whereas the concentration of free carnitine was significantly lower at term than at the 20th week of pregnancy (16.75 +/- 0.89 versus 19.61 +/- 1.25). Almost all of C2- to C12-carnitine esters were significantly lower, whereas C16- and C18-carnitines with in-chain modifications were significantly higher in mothers at delivery compared with nonpregnant women. Plasma levels of free carnitine and C2-, C3-, C4-, C5-, C6-, and C16-carnitines were significantly lower, while concentrations of carnitine esters with 8, 10, 12 and 18 carbons in the acyl chain as well as C14:1-, C14:2-, and C16:1-OH-carnitines were significantly higher in mothers at term than in their neonates. The data of the present study clearly show dynamic features of plasma carnitine profile during pregnancy and indicate an extraordinarily active participation of the carnitine in the intermediary metabolism both in the pregnant woman and in the neonate.
我们采用电喷雾电离（ESI）串联质谱法，对37名孕妇在妊娠第20周、第30周和分娩时，以及她们的新生儿出生时，以及22名年龄匹配的非孕妇的血浆中游离肉碱和30种肉碱酯的浓度进行了研究。足月时，乙酰肉碱和含五个以上碳原子的肉碱酯的血浆水平显著升高，而游离肉碱的浓度显著低于妊娠第20周时（16.75±0.89对19.61±1.25）。与非孕妇相比，几乎所有C2至C12肉碱酯在分娩时的母亲中显著降低，而具有链内修饰的C16和C18肉碱则显著升高。足月时母亲血浆中游离肉碱以及C2、C3、C4、C5、C6和C16肉碱的水平显著降低，而酰基链中含8、10、12和18个碳原子的肉碱酯以及C14:1-、C14:2-和C16:1-OH-肉碱的浓度在足月时母亲中显著高于其新生儿。本研究数据清楚地显示了孕期血浆肉碱谱的动态特征，并表明肉碱在孕妇和新生儿的中间代谢中都有非常活跃的参与。
