Song Yiqing, Lyu Chen, Li Ming, Rahman Mohammad L, Chen Zhen, Zhu Yeyi, Hinkle Stefanie N, Chen Liwei, Mitro Susanna D, Li Ling-Jun, Weir Natalie L, Tsai Michael Y, Zhang Cuilin
Department of Epidemiology, Indiana University Richard M. Fairbanks School of Public Health, Indianapolis, IN 46202, USA.
Department of Population Health, Division of Biostatistics, NYU Grossman School of Medicine, NYU Langone Health, New York, NY 10016, USA.
Metabolites. 2021 Dec 17;11(12):885. doi: 10.3390/metabo11120885.
As surrogate readouts reflecting mitochondrial dysfunction, elevated levels of plasma acylcarnitines have been associated with cardiometabolic disorders, such as obesity, gestational diabetes, and type 2 diabetes. This study aimed to examine prospective associations of acylcarnitine profiles across gestation with neonatal anthropometry, including birthweight, birthweight z score, body length, sum of skinfolds, and sum of body circumferences. We quantified 28 acylcarnitines using electrospray ionization tandem mass spectrometry in plasma collected at gestational weeks 10-14, 15-26, 23-31, and 33-39 among 321 pregnant women from the National Institute of Child Health and Human Development (NICHD) Fetal Growth Studies-Singletons. A latent-class trajectory approach was applied to identify trajectories of acylcarnitines across gestation. We examined the associations of individual acylcarnitines and distinct trajectory groups with neonatal anthropometry using weighted generalized linear models adjusting for maternal age, race/ethnicity, education, parity, gestational age at blood collection, and pre-pregnancy body mass index (BMI). We identified three distinct trajectory groups in C2, C3, and C4 and two trajectory groups in C5, C10, C5-DC, C8:1, C10:1, and C12, respectively. Women with nonlinear decreasing C12 levels across gestation (5.7%) had offspring with significantly lower birthweight (-475 g; 95% CI, -942, -6.79), birthweight z score (-0.39, -0.71, -0.06), and birth length (-1.38 cm, -2.49, -0.27) than those with persistently stable C12 levels (94.3%) (all nominal value < 0.05). Women with consistently higher levels of C10 (6.1%) had offspring with thicker sum of skinfolds (4.91 mm, 0.85, 8.98) than did women with lower levels (93.9%) during pregnancy, whereas women with lower C10:1 levels (12.6%) had offspring with thicker sum of skinfolds (3.23 mm, 0.19, 6.27) than did women with abruptly increasing levels (87.4%) ( < 0.05). In conclusion, this study suggests that distinctive trajectories of C10, C10:1, and C12 acylcarnitine levels throughout pregnancy were significantly associated with neonatal anthropometry.
作为反映线粒体功能障碍的替代指标，血浆酰基肉碱水平升高与肥胖、妊娠期糖尿病和2型糖尿病等心脏代谢紊乱有关。本研究旨在探讨孕期酰基肉碱谱与新生儿人体测量指标（包括出生体重、出生体重z评分、身长、皮褶厚度总和及体围总和）之间的前瞻性关联。我们使用电喷雾电离串联质谱法对来自美国国立儿童健康与人类发展研究所（NICHD）单胎胎儿生长研究的321名孕妇在孕10 - 14周、15 - 26周、23 - 31周和33 - 39周采集的血浆中的28种酰基肉碱进行了定量。采用潜在类别轨迹法来识别孕期酰基肉碱的轨迹。我们使用加权广义线性模型，对母亲年龄、种族/族裔、教育程度、产次、采血时的孕周和孕前体重指数（BMI）进行调整，研究了个体酰基肉碱和不同轨迹组与新生儿人体测量指标之间的关联。我们分别在C2、C3和C4中识别出三个不同的轨迹组，在C5、C10、C5 - DC、C8:1、C10:1和C12中识别出两个轨迹组。孕期C12水平呈非线性下降的女性（5.7%）所生后代的出生体重（-475 g；95% CI，-942，-6.79）、出生体重z评分（-0.39，-0.71，-0.06）和出生身长（-1.38 cm，-2.49，-0.27）显著低于C12水平持续稳定的女性（94.3%）（所有P值< 0.05）。孕期C10水平持续较高的女性（6.1%）所生后代的皮褶厚度总和（4.91 mm，0.85，8.98）比孕期C10水平较低的女性（93.9%）更厚，而孕期C10:1水平较低的女性（- 12.6%）所生后代的皮褶厚度总和（3.23 mm，0.19，6.27）比C10:1水平突然升高的女性（87.4%）更厚（P < 0.05）。总之，本研究表明孕期C10、C10:1和C12酰基肉碱水平的独特轨迹与新生儿人体测量指标显著相关。
