Van Ewijk Bart E, Wolfs Tom F W, Aerts Piet C, Van Kessel Kok P M, Fleer Andre, Kimpen Jan L L, Van der Ent Cornelis K
Cystic Fibrosis Centre and Department of Pediatric Respiratory Medicine, Wilhelmina Children's Hospital, University Medical Centre Utrecht, Utrecht, Netherlands.
Pediatr Res. 2007 Apr;61(4):398-403. doi: 10.1203/pdr.0b013e3180332d1c.
Cystic fibrosis lung disease typically has a course of exacerbations and remissions, suggesting that external factors like viral infections can influence this course. Clinical data suggest synergism between respiratory syncytial virus (RSV) infections and Pseudomonas aeruginosa in cystic fibrosis (CF) lung disease. We studied the influence of RSV infection on adherence of P. aeruginosa to IB3-1, HEp-2, and A549 epithelial cell monolayers in vitro. RSV infection of epithelial cells as well as simultaneous addition of RSV and P. aeruginosa to noninfected cells both strongly enhanced the pseudomonal adherence to epithelial cells. The increased adherence varied from 1.2- to 8.2-fold in case of previous RSV infection, and from 1.7- to 16.1-fold in case of simultaneous addition. We observed direct binding of RSV to P. aeruginosa, and blocking of RSV with heparin eliminated the effect on increased adherence. This suggests that RSV possibly acts as a coupling agent between P. aeruginosa and epithelial cells. In conclusion, RSV enhances P. aeruginosa infection of respiratory epithelial cells. It suggests a role of specific viral-bacterial interactions in exacerbations of CF lung disease, which could have important implications on prevention and treatment.
囊性纤维化肺部疾病通常呈病情加重与缓解交替的病程，这表明诸如病毒感染等外部因素会影响该病程。临床数据显示，呼吸道合胞病毒（RSV）感染与铜绿假单胞菌在囊性纤维化（CF）肺部疾病中存在协同作用。我们在体外研究了RSV感染对铜绿假单胞菌黏附于IB3-1、HEp-2和A549上皮细胞单层的影响。上皮细胞的RSV感染以及将RSV与铜绿假单胞菌同时添加至未感染细胞均显著增强了假单胞菌对上皮细胞的黏附。既往RSV感染时，黏附增加幅度为1.2至8.2倍；同时添加时，黏附增加幅度为1.7至16.1倍。我们观察到RSV与铜绿假单胞菌存在直接结合，用肝素阻断RSV可消除对黏附增加的影响。这表明RSV可能作为铜绿假单胞菌与上皮细胞之间的偶联剂。总之，RSV增强了呼吸道上皮细胞的铜绿假单胞菌感染。这提示特定病毒-细菌相互作用在CF肺部疾病加重过程中发挥作用，这可能对预防和治疗具有重要意义。
