Baechler Emily C, Bauer Jason W, Slattery Catherine A, Ortmann Ward A, Espe Karl J, Novitzke Jill, Ytterberg Steven R, Gregersen Peter K, Behrens Timothy W, Reed Ann M
University of Minnesota Medical School, Minneapolis, MN 55455, USA.
Mol Med. 2007 Jan-Feb;13(1-2):59-68. doi: 10.2119/2006-00085.Baechler.
Recent studies have shown increased expression of interferon (IFN)-regulated genes in the peripheral blood cells of patients with systemic lupus erythematosus. A similar interferon signature has been observed in affected muscle tissue from patients with dermatomyositis (DM), but it has not yet been determined if this signature extends to the peripheral blood in DM. We performed global gene expression profiling of peripheral blood cells from adult and juvenile DM patients and healthy controls. Several interesting groups of genes were differentially expressed in DM, including genes with immune function, and others that function in muscle or are involved in mitochondrial/oxidative phosphorylation. Investigation of type I IFN-regulated transcripts revealed a striking interferon signature present in most DM patients studied. Levels of type I IFN-regulated proteins were also elevated in DM serum samples. Furthermore, both the transcript and serum protein IFN signatures were associated with disease activity. These data suggest that the IFN signature may be a useful marker for DM disease activity, and that sampling peripheral blood may be a more practical alternative to muscle biopsy for measuring this signature.
近期研究表明，系统性红斑狼疮患者外周血细胞中干扰素（IFN）调节基因的表达增加。在皮肌炎（DM）患者的受累肌肉组织中也观察到了类似的干扰素特征，但这种特征是否延伸至DM患者的外周血尚未确定。我们对成年和青少年DM患者及健康对照者的外周血细胞进行了全基因组表达谱分析。在DM中，有几组有趣的基因存在差异表达，包括具有免疫功能的基因，以及在肌肉中发挥作用或参与线粒体/氧化磷酸化的其他基因。对I型干扰素调节转录本的研究发现，在大多数研究的DM患者中存在显著的干扰素特征。DM血清样本中I型干扰素调节蛋白的水平也升高。此外，转录本和血清蛋白干扰素特征均与疾病活动相关。这些数据表明，干扰素特征可能是DM疾病活动的有用标志物，并且对于测量这种特征，采集外周血可能是比肌肉活检更实用的替代方法。
