SIVmac32H J5病毒在体内快速连续传代后新攻击毒株的制备与鉴定
Preparation and characterization of new challenge stocks of SIVmac32H J5 following rapid serial passage of virus in vivo.
作者信息
Ferguson D, Wade-Evans A, Elsley W, Sangster R, Silvera P, MacManus S, Davis G, Corcoran T, Berry N, Brown S, Jenkins A, Cowie J, Sethi M, Hull R, Stebbings R, Lines J, Norley S, Stott E J, Almond N
机构信息
Division of Retrovirology, NIBSC, South Mimms, Potters Bar, Hertfordshire, UK.
出版信息
J Med Primatol. 2007 Jun;36(3):131-42. doi: 10.1111/j.1600-0684.2007.00224.x.
BACKGROUND
A new challenge stock of the simian immunodeficiency virus SIVmacJ5 has been produced following passage in vivo.
METHODS
SIVmacJ5 3/92 (J5M), was passaged serially through cynomolgus macaques (Macaca fascicularis) by intravenous inoculation of infected spleen cells isolated and prepared 14 days post-infection. Two challenge stocks, SIVmacJ5 S61MLN and SIVmacJ5 S62spl, were prepared by culture of lymphoid tissue ex vivo.
RESULTS
These virus stocks appeared better adapted for replication in M. fascicularis as demonstrated by a greater persistence of recoverable live virus from the periphery and increased pathology in lymphoid tissues 20 weeks post-challenge as detected by immunohistochemistry. Sequence analysis of the envelope gene from these stocks did not identify marked diversification of sequence as a result of this procedure.
CONCLUSIONS
These stocks display more robust peripheral persistence and tissue pathology in cynomolgus macaques and should prove valuable analysing recombinant vaccines based upon SIVmacJ5 transgenes.
背景
在体内传代后制备了一种新的猴免疫缺陷病毒SIVmacJ5攻击用毒株。
方法
通过静脉接种感染后14天分离并制备的感染脾细胞，将SIVmacJ5 3/92（J5M）在食蟹猴（猕猴属）中连续传代。通过体外培养淋巴组织制备了两种攻击用毒株，即SIVmacJ5 S61MLN和SIVmacJ5 S62spl。
结果
这些病毒毒株似乎更适合在食蟹猴中复制，这表现为外周可恢复的活病毒持续时间更长，且在攻击后20周通过免疫组织化学检测发现淋巴组织中的病理学变化增加。对这些毒株的包膜基因进行序列分析未发现该过程导致序列出现明显多样化。
结论
这些毒株在食蟹猴中表现出更强的外周持续性和组织病理学变化，在分析基于SIVmacJ5转基因的重组疫苗方面应具有重要价值。
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