Leibo S P, Kubisch H Michael, Schramm R Dee, Harrison Richard M, VandeVoort Catherine A
Department of Biological Sciences, University of New Orleans, Audubon Center for Research of Endangered Species, New Orleans, LA, USA.
J Med Primatol. 2007 Jun;36(3):151-63. doi: 10.1111/j.1600-0684.2006.00194.x.
The efficiency of controlled propagation to produce rhesus monkeys of particular genotypes can be maximized by use of cryopreserved spermatozoa collected from specific males to inseminate appropriate females. But this assumes that semen from males with different genotypes can be cryopreserved with equal effectiveness.
To investigate whether spermatozoa from different Macaca mulatta males can be effectively cryopreserved when frozen under identical conditions, we collected and froze semen specimens from 13 adult, fertile males maintained at three primate research centers.
Survival, based on post-thaw motility normalized to the pre-freeze value, was assayed within 30 minutes after thawing; it varied from 50% to 70% but declined thereafter. To examine the response of semen from individual males, we collected and froze three to six ejaculates per male from each of seven males.
In general, semen from a given male responded reproducibly to freezing, but there were significant differences among males. The cause of these differences among M. mulatta males in post-thaw sperm survival remains unidentified.
通过使用从特定雄性猕猴采集的冷冻精子对合适的雌性猕猴进行授精，可以最大限度地提高繁殖特定基因型猕猴的效率。但这假定来自不同基因型雄性的精液能够以相同的效果进行冷冻保存。
为了研究在相同条件下冷冻时，来自不同恒河猴雄性的精子是否能被有效冷冻保存，我们从三个灵长类动物研究中心饲养的13只成年可育雄性猕猴采集并冷冻精液样本。
解冻后30分钟内测定了基于解冻后活力相对于冷冻前值的精子存活率；存活率在50%至70%之间变化，但此后下降。为了研究个体雄性猕猴精液的反应，我们从7只雄性猕猴中的每只采集并冷冻了三到六次射精样本。
总体而言，来自特定雄性的精液对冷冻的反应具有可重复性，但不同雄性之间存在显著差异。恒河猴雄性解冻后精子存活率存在这些差异的原因尚不清楚。
