Yang Huiping, Tiersch Terrence R
Aquaculture Research Station, Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, 2410 Ben Hur Road, Baton Rouge, Louisiana 70820, USA.
Comp Biochem Physiol C Toxicol Pharmacol. 2009 Mar;149(2):224-32. doi: 10.1016/j.cbpc.2008.07.005. Epub 2008 Jul 18.
Aquarium fishes are becoming increasingly important because of their value in biomedical research and the ornamental fish trade, and because many have become threatened or endangered in the wild. This review summarizes the current status of sperm cryopreservation in three fishes widely used in biomedical research: zebrafish, medaka, and live-bearing fishes of the genus Xiphophorus, and will focus on the needs and opportunities for future research and application of cryopreservation in aquarium fish. First, we summarize the basic biological characteristics regarding natural habitat, testis structure, spermatogenesis, sperm morphology, and sperm physiology. Second, we compare protocol development of sperm cryopreservation. Third, we emphasize the importance of artificial fertilization in sperm cryopreservation to evaluate the viability of thawed sperm. We conclude with a look to future research directions for sperm cryopreservation and the application of this technique in aquarium species.
由于水族馆鱼类在生物医学研究和观赏鱼贸易中的价值，以及许多野生水族馆鱼类受到威胁或濒临灭绝，它们正变得越来越重要。本综述总结了生物医学研究中广泛使用的三种鱼类（斑马鱼、青鳉和剑尾鱼属的卵胎生鱼类）精子冷冻保存的现状，并将重点关注水族馆鱼类冷冻保存未来研究和应用的需求与机遇。首先，我们总结了有关自然栖息地、睾丸结构、精子发生、精子形态和精子生理学的基本生物学特征。其次，我们比较了精子冷冻保存方案的制定。第三，我们强调人工授精在精子冷冻保存中对于评估解冻精子活力的重要性。最后，我们展望了精子冷冻保存的未来研究方向以及该技术在水族馆物种中的应用。
