Oktay Kutluk, Demirtas Ezgi, Son Weon-Young, Lostritto Kathy, Chian Ri-Cheng, Tan Seang Lin
Department of Obstetrics and Gynecology, Center for Reproductive Medicine and Infertility, Weill Medical College, Cornell University, New York, New York 10021, USA.
Fertil Steril. 2008 Jan;89(1):228.e19-22. doi: 10.1016/j.fertnstert.2007.02.028. Epub 2007 May 22.
To report a novel approach to fertility preservation by in vitro maturation and embryo cryopreservation after LH surge and ovulation.
Case report.
Two university-based reproductive endocrinology clinics.
PATIENT(S): Forty-year-old woman with breast cancer seeking fertility preservation before chemotherapy.
INTERVENTION(S): The plan was ovarian stimulation and retrieval of mature oocytes, followed by IVF; however, premature LH surge and ovulation occurred before oocyte retrieval. Because no mature oocyte was recovered, follicles <10 mm in diameter were also aspirated.
MAIN OUTCOME MEASURE(S): Recovery of immature oocytes after ovulation, in vitro maturation of these oocytes, and fertilization and vitrification of generated embryos.
RESULT(S): Four immature oocytes were recovered, and two matured in vitro. After fertilization with intracytoplasmic sperm injection, embryos progressed to the four-cell stage on day 2 and were vitrified for future use.
CONCLUSION(S): This case illustrates that under time constraints, immature oocytes can be recovered after ovulation and used to generate embryos for fertility preservation.
报告一种在促黄体生成素峰和排卵后通过体外成熟和胚胎冷冻保存来保留生育能力的新方法。
病例报告。
两家大学附属生殖内分泌诊所。
一名40岁乳腺癌女性，在化疗前寻求生育力保存。
计划是进行卵巢刺激并获取成熟卵母细胞，随后进行体外受精；然而，在获取卵母细胞前出现了过早的促黄体生成素峰和排卵。由于未回收成熟卵母细胞，直径<10 mm的卵泡也被抽吸。
排卵后未成熟卵母细胞的回收、这些卵母细胞的体外成熟以及所产生胚胎的受精和玻璃化。
回收了4个未成熟卵母细胞，其中2个在体外成熟。经卵胞浆内单精子注射受精后，胚胎在第2天发育至四细胞阶段并被玻璃化以备将来使用。
本病例表明，在时间紧迫的情况下，排卵后可回收未成熟卵母细胞并用于生成胚胎以保存生育能力。
