Weiss Amir, Neril Rebecca, Geslevich Joel, Lavee Michal, Beck-Fruchter Ronit, Golan Joanne, Shalev Eliezer
Fertility and In Vitro Fertilization Unit, Department of Obstetrics and Gynecology, Emek Medical Center, Afula, Israel.
Rappaport School of Medicine, Technion-Israel Institute of Technology, Haifa, Israel.
Fertil Steril. 2014 Aug;102(2):419-23. doi: 10.1016/j.fertnstert.2014.04.041. Epub 2014 May 28.
To study the correlation between the lag time from ovulation trigger to oocyte aspiration and the proportion of metaphase II (MII) mature oocytes aspirated.
Retrospective study.
Fertility and IVF center in an academic regional hospital.
PATIENT(S): A total of 511 women undergoing IVF-intracytoplasmic sperm injection at our center, with at least one oocyte available for maturity determination.
INTERVENTION(S): Data were retrieved from patient electronic databases and files. Demographic data, hormone treatments and ovarian response, and the time of ovulation trigger and oocyte aspiration were recorded.
MAIN OUTCOME MEASURE(S): The primary outcome was the proportion of MII mature oocytes relative to the total number of oocytes aspirated and allocated to intracytoplasmic sperm injection. Pregnancy rates and clinical pregnancy rates were secondary outcomes.
RESULT(S): There were fewer MII mature oocytes when the lag time between oocyte trigger and aspiration was between 33.45 hours and 34.45 hours. The proportion of MII oocytes seems to increase up to a 35-hour lag time and then stabilizes up to 38 hours. Pregnancy and clinical pregnancy rates did not differ among the different time groups studied.
CONCLUSION(S): Oocyte aspiration should be scheduled at least 35 hours after ovulation trigger. Oocytes can be aspirated in a 3-hour window of time between 35 and 38 hours without compromising results. Further research should elucidate whether even longer lag times will improve the proportion of MII mature oocytes.
研究从排卵触发到卵母细胞抽吸的间隔时间与抽吸到的中期II（MII）期成熟卵母细胞比例之间的相关性。
回顾性研究。
某地区学术医院的生殖与体外受精中心。
共有511名在本中心接受体外受精-卵胞浆内单精子注射的女性，至少有一个卵母细胞可用于成熟度测定。
从患者电子数据库和档案中检索数据。记录人口统计学数据、激素治疗及卵巢反应，以及排卵触发时间和卵母细胞抽吸时间。
主要观察指标是MII期成熟卵母细胞占抽吸并分配用于卵胞浆内单精子注射的卵母细胞总数的比例。妊娠率和临床妊娠率为次要观察指标。
当卵母细胞触发与抽吸之间的间隔时间在33.45小时至34.45小时之间时，MII期成熟卵母细胞较少。MII期卵母细胞比例似乎在间隔时间达到35小时之前增加，然后在38小时内保持稳定。在所研究的不同时间组中，妊娠率和临床妊娠率没有差异。
卵母细胞抽吸应安排在排卵触发后至少35小时进行。在35至38小时的3小时时间窗口内可以抽吸卵母细胞，而不会影响结果。进一步的研究应阐明更长的间隔时间是否会提高MII期成熟卵母细胞的比例。
