Strelkauskas A J, Aldenderfer P H, Warner G A
Department of Microbiology and Immunology, Medical University of South Carolina, Charleston 29405.
Hum Antibodies Hybridomas. 1991 Oct;2(4):207-14.
The human monoclonal antibody JDB1 was examined for reactivity to five breast cancer cell lines as well as normal fibroblasts. The JDB1 clone secretes both IgG3 and IgM antibody. In these studies both the total antibody (containing IgG3 and IgM) as well as the purified IgG3 and IgM fractions were examined for tumor cell binding reactivity. Peroxidase staining was observed in the breast cancer lines SW527, MCF-7, T47D, SKBR3, and MDAMB231, while GM179 and GM5758 fibroblast lines were negative for antibody binding. Tumor cells were examined using two techniques: a drop cell technique in which cells were fixed onto slides and also a cover slip assay in which cells were grown onto sterile cover slips and subsequently stained with the human monoclonal antibody using a peroxidase technique. Both cytoplasmic and membrane staining were observed for all of the breast tumor cells tested using both assays. In addition, a cellular ELISA was developed and used to quantitate the binding of these antibodies to tumor cells.
检测了人源单克隆抗体JDB1对五种乳腺癌细胞系以及正常成纤维细胞的反应性。JDB1克隆分泌IgG3和IgM抗体。在这些研究中，检测了总抗体（包含IgG3和IgM）以及纯化的IgG3和IgM组分与肿瘤细胞的结合反应性。在乳腺癌细胞系SW527、MCF - 7、T47D、SKBR3和MDAMB231中观察到过氧化物酶染色，而GM179和GM5758成纤维细胞系的抗体结合呈阴性。使用两种技术检测肿瘤细胞：一种是将细胞固定在载玻片上的滴片细胞技术，另一种是将细胞生长在无菌盖玻片上，随后使用过氧化物酶技术用人源单克隆抗体进行染色的盖玻片检测法。使用这两种检测方法对所有测试的乳腺肿瘤细胞均观察到细胞质和膜染色。此外，还开发了一种细胞ELISA并用于定量这些抗体与肿瘤细胞的结合。
