Strelkauskas A J, Welsh J, Metcalf J S, Aldenderfer P H
Department of Microbiology and Immunology, Medical University of South Carolina, Charleston 29425, USA.
Hum Antibodies Hybridomas. 1994;5(3-4):157-64.
Numerous murine monoclonal antibodies reactive with human mammary tumor cells have been developed and used for identification of various breast tumor antigens. The problems encountered with these murine monoclonal antibodies include poor sensitivity and cross reactivity with normal tissue. We report here the use of a human monoclonal antibody (JDB1) as a more sensitive and specific method for staining of paraffin-embedded breast carcinoma tissue. This human clone was constructed by fusing the human lymphoma cell line (Ball-1) and peripheral blood lymphocytes from a breast cancer patient in active immunological remission of her disease. Several benign and malignant breast lesions in addition to a variety of apparently normal human tissues have been stained. To date, a variety of breast carcinomas have stained positively with JDB1, while normal breast tissue samples as well as other forms of malignant tissue were negative for staining. In addition, unlike most commercially available murine monoclonals, JDB1 was able to identify metastatic infiltrating ductal carcinoma to both axillary nodes and skin. These results represent one of the first reported findings of immunostaining using a human monoclonal antibody on paraffin-embedded formalin-fixed human breast carcinoma. These human antibodies provide new research tools with which to evaluate human breast cancer and allow for a more complete understanding of the heterogeneity associated with this disease.
已经开发出许多与人类乳腺肿瘤细胞反应的鼠单克隆抗体，并用于鉴定各种乳腺肿瘤抗原。这些鼠单克隆抗体遇到的问题包括灵敏度低以及与正常组织的交叉反应性。我们在此报告使用人单克隆抗体（JDB1）作为石蜡包埋乳腺癌组织染色的一种更灵敏且特异的方法。该人源克隆是通过将人淋巴瘤细胞系（Ball-1）与来自一名处于疾病主动免疫缓解期的乳腺癌患者的外周血淋巴细胞融合构建而成。除了各种明显正常的人体组织外，还对几种良性和恶性乳腺病变进行了染色。迄今为止，多种乳腺癌用JDB1染色呈阳性，而正常乳腺组织样本以及其他形式的恶性组织染色均为阴性。此外，与大多数市售鼠单克隆抗体不同，JDB1能够识别转移至腋窝淋巴结和皮肤的浸润性导管癌。这些结果代表了首次报道的使用人单克隆抗体对石蜡包埋的福尔马林固定的人乳腺癌进行免疫染色的发现之一。这些人源抗体提供了新的研究工具，可用于评估人类乳腺癌，并有助于更全面地了解与该疾病相关的异质性。
