[气相色谱-质谱联用及化学计量学解析法分析麻黄桂枝挥发油]

[Analysis of essential oil in herba ephedrae-ramulus cinnamomi by GC-MS and chemometric resolution method].

作者信息

Li Xiao-ru, Liang Yi-zeng, Li Xiao-ning

机构信息

College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.

出版信息

Yao Xue Xue Bao. 2007 Feb;42(2):187-91.

PMID:17518049

Abstract

Gas chromatography/mass spectrometry, chemometric resolution method ( CRM) and overall volume integration method were used to analyze the essential components of herbal pair Herba Ephedrae-Ramulus Cinnamomi (HP HE-RC) and compare it with that of single herbs HE and RC. 72, 68, and 97 essential components in essential oil of HE, RC, and HP HE-RC were determined, accounting for 90. 08% , 91. 62% , and 89. 76% of total contents of essential oil of HE, RC, and HP HE-RC, respectively. The numbers of essential components of HP HE-RC are almost the summation of that of two single herbs, but some relative contents of them are changed. Some new components, such as 1, 6-dimethylhepta-1,3,5-triene, tetracyclo[4. 2. 1.1 (2,5 ). 0 (9,10) ] deca-3,7-diene, globulol, ( E, E) -6, 10,14-trimethyl-5,9,13-pentadecatrien-2 -one, etc. have been found in HP HE-RC because of chemical reactions and physical changes during the course of decocation.

摘要

采用气相色谱/质谱联用技术、化学计量学分辨方法（CRM）和总体积积分法对药对麻黄-桂枝（HP HE-RC）的挥发油主要成分进行分析，并与单味药麻黄（HE）和桂枝（RC）进行比较。分别测定了HE、RC和HP HE-RC挥发油中的72、68和97种主要成分，分别占HE、RC和HP HE-RC挥发油总含量的90.08%、91.62%和89.76%。HP HE-RC的主要成分数量几乎是两种单味药的总和，但其中一些成分的相对含量发生了变化。由于煎煮过程中的化学反应和物理变化，在HP HE-RC中发现了一些新成分，如1,6-二甲基-1,3,5-庚三烯、四环[4.2.1.1(2,5).0(9,10)]癸-3,7-二烯、球松素、(E,E)-6,10,14-三甲基-5,9,13-十五碳三烯-2-酮等。