Shi Yue, Shi Ren-bing
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100094, China.
Yao Xue Xue Bao. 2007 Feb;42(2):192-6.
HPLC and LC-MS/MS were used to establish a comprehensive HPLC analytical method of Yinqiao decoction and identify the chemical constituents of the whole and individual herbs of Yinqiao decoction. YWG-C18 (250 mm x4. 6 mm ID, 10 microm) column was used; the mobile phase was composed of acetonitrile (A) and water ( B, with 3% acetic acid) with gradient elution; the flow rate was 1. 0 mL x min(-1) and the column temperature was set up at 25 degrees C. The detection wavelength was 280 nm. The chromatographic fingerprints of Yinqiao Decoction showed 30 main peaks. Peak 2, 14, 15, 17 were from Lonicera japonica Thunb, peak 3, 12, 13, 24 were from Fosythia suspense (Thunb) Vahl, peak 19, 25, 26, 27 were from Arctium lappa L. , peak 5, 6, 8, 9, 10, 11, 18, 28 were from Glycyrrhiza uralensis Fisch, peak 20, 21 were from Mentha haplocalyx Briq. , peak 22, 23 were from Schizonepeta tenuifolia Briq. , peak 1 presented in the chromatograms of Lonicera japonica Thunb, Fosythia suspense (Thunb) Vahl, Mentha haplocalyx Briq. , Schizonepeta tenuifolia Briq. and Platycodon grandiflorum (Jacq. ) A. DC. , peak 7 presented in the chromatograms of Fosythia suspense (Thunb) Vahl and Glycine max (L. ) Merr. , peak 16 presented in the chromatograms of Mentha haplocalyx Briq. and Schizonepeta tenuifolia Briq. , peak 29 presented in the chromatograms of the herbs except Mentha haplocalyx Briq. and Platycodon grandiflorum (Jacq. ) A. DC. , peak 30 presented in the chromatograms of the herbs except Platycodon grandiflorum (Jacq. ) A. DC. , peak 4 was not identified, maybe it was a new constituent produced during decoction. By comparison of the standards isolated and MS spectra, 14 peaks were identified as 2 ( chlorogenic acid) , 9 ( liquiritin ) , 10 ( 4'-O-[ beta-D-apiofuranosyl (1--> 2 ) -beta-D-glucopyranosyl] liquiritigenin), 12 (forsythiaside), 13 (rutin), 14 (4,5-O-dicaffeoylquiniic acid), 15 (3, 5-O-dicaffeoylquiniic acid ), 16 ( 4-0- [ beta-D-apiofuranosyl ( 1 -->2 ) -beta-D-glucopyranosyl ] isoliquiritigenin) , 17 ( 3, 4-O-dicaffeoylquiniic acid) , 18 (2'-O-[ beta-D-apiofuranosyl (1 -->2 ) -beta-D-glucopyranosyl] isoliquiritigenin) , 19 (arctiin) , 20 (linarin) , 25 (genistein) , 28 ( isoliquiritigenin) . The method could be used to identify the characteristics of Yinqiao decoction, and it could be used to evaluate the quality and quantity of Yinqiao decoction.
采用高效液相色谱法（HPLC）和液相色谱 - 串联质谱法（LC - MS/MS）建立银翘汤的HPLC综合分析方法，并鉴定银翘汤全方及单味药的化学成分。采用YWG - C18（250 mm×4.6 mm内径，10μm）色谱柱；流动相由乙腈（A）和水（B，含3%乙酸）组成，梯度洗脱；流速为1.0 mL·min⁻¹，柱温设定为25℃。检测波长为280 nm。银翘汤的色谱指纹图谱显示有30个主峰。峰2、14、15、17来自金银花，峰3、12、13、24来自连翘，峰19、25、26、27来自牛蒡子，峰5、6、8、9、10、11、18、28来自甘草，峰20、21来自薄荷，峰22、23来自荆芥，峰1出现在金银花、连翘、薄荷、荆芥和桔梗的色谱图中；峰7出现在连翘和大豆的色谱图中；峰16出现在薄荷和荆芥的色谱图中；峰29出现在除薄荷和桔梗以外的药材色谱图中；峰30出现在除桔梗以外的药材色谱图中；峰4未鉴定出来，可能是煎煮过程中产生的新成分。通过与分离得到的对照品和质谱图比较，鉴定出14个峰分别为2（绿原酸）、9（甘草苷）、10（4'-O - [β - D - 芹菜糖基（1→2）-β - D - 葡萄糖基]甘草素）、12（连翘苷）、13（芦丁）、14（4,5 - O - 二咖啡酰奎宁酸）、15（3,5 - O - 二咖啡酰奎宁酸）、16（4 - O - [β - D - 芹菜糖基（1→2）-β - D - 葡萄糖基]异甘草素）、17（3,4 - O - 二咖啡酰奎宁酸）；18（2'-O - [β - D - 芹菜糖基（1→2）-β - D - 葡萄糖基]异甘草素）、19（牛蒡子苷）、20（蒙花苷）、25（染料木素）、28（异甘草素）。该方法可用于鉴定银翘汤的特征，也可用于评价银翘汤的质量和含量。
