Yu Bing-Sheng, Yan Xiao-Pin, Xiong Jingyu, Xin Qi
Department of Chemistry, Zhanjiang Normal College, China.
Chem Pharm Bull (Tokyo). 2003 Apr;51(4):421-4. doi: 10.1248/cpb.51.421.
A reversed phase (RP)-HPLC method was established for simultaneous determination of chlorogenic acid, arctiin and forsythin in Yinqiao Jiedu Granules, which was a commonly used Chinese herbal medical preparation for treatment of rheum ailments. The determination was based on a gradient elution (A: 1% acetic acid, pH=3.0, B: methanol) on a C18 column and an automatic wavelength switching program, where 325 nm was used for chlorogenic acid and 280 nm for arctiin and forsythin, respectively. Good linearities were obtained over the range of 2-200 mg.l(-1) for the 3 objective compounds. The spike recoveries were within 96.0-97.9%.
建立了一种反相（RP）-高效液相色谱法，用于同时测定银翘解毒颗粒中的绿原酸、牛蒡子苷和连翘苷。银翘解毒颗粒是一种常用的治疗风湿疾病的中草药制剂。该测定基于在C18柱上的梯度洗脱（A：1%乙酸，pH = 3.0，B：甲醇）和自动波长切换程序，其中绿原酸采用325 nm波长，牛蒡子苷和连翘苷分别采用280 nm波长。3种目标化合物在2 - 200 mg·l⁻¹范围内具有良好的线性关系。加样回收率在96.0% - 97.9%之间。
